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Related Experiment Video

Updated: Aug 26, 2025

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

Isotope Ratio Encoding of Sequence-Defined Oligomers.

Márton Zwillinger^1,2, Lucile Fischer³, Gergő Sályi¹

¹Servier Research Institute of Medicinal Chemistry, H-1031 Budapest, Hungary.

Journal of the American Chemical Society

|October 7, 2022

Summary

Scientists developed a novel isotope ratio encoding method using deuterium-labeled monomers to create unique molecular fingerprints. This technique allows for high-fidelity information readout via mass spectrometry, distinguishing complex molecular structures.

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Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

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Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

Published on: November 14, 2017

Area of Science:

Molecular Biology
Analytical Chemistry
Biotechnology

Background:

Molecular information storage often relies on ordered monomer sequences and mass spectrometry.
Current methods may involve complex fragmentation or mixtures of molecules for data retrieval.

Purpose of the Study:

To introduce an alternative isotope ratio encoding strategy for molecular information storage.
To demonstrate the capability of distinguishing oligomers based on their unique isotope distribution patterns.

Main Methods:

Utilizing deuterium-labeled monomers to synthesize hundreds of unique oligomers.
Employing mass spectrometry to recognize distinct isotope distribution patterns for information readout.
Distinguishing all 256 tetramers composed of four different monomers using deuterated building blocks.

Main Results:

Successfully encoded and decoded information in oligomers with high fidelity using isotope ratio patterns.
Demonstrated the ability to differentiate tetramers based on their mass fingerprints derived from mono-, di-, tri-, and tetradeuterated monomers.
Confirmed the method's robustness against experimental errors and its compatibility with standard mass spectrometry instrumentation.

Conclusions:

Isotope ratio encoding offers a direct method for writing information into molecular compounds via isotopologue distribution.
This approach obviates the need for additional tagging or the use of molecular mixtures.
The technique holds potential for applications in molecular identity encoding and advanced data storage.