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Recombinant Protein L: Production, Purification and Characterization of a Universal Binding Ligand
Stefan Kittler1, Julian Ebner1, Mihail Besleaga2
1Research Division Integrated Bioprocess Development, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Gumpendorfer Strasse 1a, 1060 Vienna, Austria; Alfred Gruber GmbH, Nordstrasse 6, 5301 Eugendorf, Austria.
Abstract:
Protein L (PpL) is a universal binding ligand that can be used for the detection and purification of antibodies and antibody fragments. Due to the unique interaction with immunoglobulin light chains, it differs from other affinity ligands, like protein A or G. However, due to its current higher market price, PpL is still scarce in applications. In this study, we investigated the recombinant production and purification of PpL and characterized the product in detail. We present a comprehensive roadmap for the production of the versatile protein PpL in E. coli.
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