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[Nucleolar vacuoles observed in rat oocytes]
Bulletin De L'Association Des Anatomistes
|June 1, 1986
Summary
Intranucleolar cavities in rat oocytes, observed via electron microscopy, appear as distinct vacuoles and interstices. Their presence is not linked to follicular atresia, despite increasing with gonadotropin stimulation.
Area of Science:
- Cell Biology
- Reproductive Biology
- Histology
Context:
- Intranucleolar cavities were previously observed in rat oocytes using light microscopy.
- Their frequency increases with gonadotropin-stimulated follicular growth.
Purpose:
- To investigate the ultrastructure of intranucleolar cavities in rat oocytes.
- To characterize the composition and potential function of these cavities.
Summary:
- Two types of cavities, "interstices" and "vacuoles," were identified within the nucleoli of rat oocytes.
- Nucleolar vacuoles are spherical, clear-cut, and do not communicate with the nucleoplasm.
- Cytochemical analysis revealed the dense fibrillar component within cavities but no basic proteins in their walls.
Impact:
- This study provides detailed ultrastructural insights into nucleolar cavities in oocytes.
- It clarifies the composition of these structures, differentiating them from nucleoplasm.
- The findings exclude a direct relationship between these vacuoles and follicular atresia.