Related Experiment Video
Updated: Aug 26, 2025
In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells
Published on: September 23, 2021
Tumor Derived Extracellular Vesicles Modulate Gene Expression in T cells
Alejandro Pando1, Christoph Schorl2, Loren D Fast3
1Warren Alpert School of Medicine at Brown University and Division of Hematology/Oncology, Rhode Island Hospital, Providence, RI, United States; Center for Biomedical Engineering, Brown University, Providence, RI, United States.
Abstract:
Extracellular vesicles (EVs) are excreted from all cells in the human body and are heterogeneous lipid bilayer particles containing proteins, RNA, DNA, and other cargo. T cells are primary players of the adaptive immune system and have a significant role in anti-cancer immunotherapies. Tumor derived EVs have diverse effects on host cells including modulating the immune response. In this study, we investigate the role acute myeloid leukemia (AML) derived EVs have on modulation of gene expression levels in three different T cell populations (CD8+, CD4+, and CD4 + CD39 + T cells). AML derived EVs modulate gene expression levels in all three cell populations with transcripts associated with major immunoregulatory pathways being significantly altered. Genes whose expression were significantly upregulated or downregulated were analyzed for gene ontogeny category expression and for the impact of the changes on specific immunoregulatory pathways. CD8 + T cells were modulated to a larger extent than other T cell populations and gene expression levels associated with proliferation and differentiation were influenced by AML-derived EVs.
Insights
Acute myeloid leukemia (AML) derived extracellular vesicles (EVs) alter gene expression in T cells, impacting immune regulation. CD8+ T cells showed significant changes in proliferation and differentiation pathways due to AML EVs.
Area of Science:
- Immunology
- Cell Biology
- Oncology
Background:
- Extracellular vesicles (EVs) are released by cells and carry various biomolecules.
- T cells are crucial for adaptive immunity and cancer immunotherapy.
- Tumor-derived EVs can influence host immune responses.
Purpose of the Study:
- To investigate how acute myeloid leukemia (AML)-derived EVs affect gene expression in different T cell populations.
- To understand the immunomodulatory impact of AML EVs on T cells.
Main Methods:
- Analysis of gene expression changes in CD8+, CD4+, and CD4+CD39+ T cells upon exposure to AML-derived EVs.
- Gene ontology analysis to identify affected pathways.
Main Results:
- AML-derived EVs significantly altered gene expression in all three T cell populations.
- Transcripts related to major immunoregulatory pathways were affected.
- CD8+ T cells exhibited more extensive modulation, with changes in proliferation and differentiation genes.
Conclusions:
- AML-derived EVs possess potent immunomodulatory capabilities.
- These EVs can significantly alter T cell function, particularly affecting CD8+ T cell proliferation and differentiation.
- Understanding these interactions is vital for developing novel AML immunotherapies.
More Related Videos
10:09Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment
Published on: June 2, 2020
11:15A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells
Published on: May 6, 2018
Related Concept Videos
01:27Tumor Immunotherapy
01:36Overview of Exosomes
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
02:17The Tumor Microenvironment
01:15Receptor Downregulation in MVBs
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...