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Study on a Natural Silk Cocoon Membrane-Based Versatile and Stable Immunosensing Platform via Directional
Hongmei Wang1,2, Shengbao Duan1,2, Yezhou Chen1,2
1CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences. No. 88, Keling Road, Suzhou, Jiangsu Province 215163, China.
ACS Omega
|October 10, 2022
Summary
A novel silk cocoon membrane serves as a stable substrate for immunosensors, overcoming challenges in biomolecule immobilization for in vitro diagnostics. This natural material offers a versatile and thermally stable platform for sensitive immunoassays.
Area of Science:
- Biomaterials Science
- Analytical Chemistry
- Immunotechnology
Background:
- Development of immunosensing assays for in vitro diagnostics faces challenges with biomolecule bioactivity and longevity on conventional substrates.
- Existing substrate materials and immobilization techniques for immunosensors often exhibit limitations in stability and effectiveness.
Purpose of the Study:
- To investigate the potential of a natural silk cocoon membrane as a substrate for immunosensing applications.
- To address the limitations of bioactivity and longevity in current immunosensor development.
- To develop a versatile and thermally stable immunosensing platform using a natural biomaterial.
Main Methods:
- Utilized a natural silk cocoon membrane as the substrate material for immunosensor development.
- Employed directional immunoaffinity recognition for immobilizing target biomolecules onto the silk membrane.
- Evaluated the immunosensor's performance for qualitative and quantitative immunoassays, including naked-eye observation and colorimetric analysis.
- Assessed the detection capability for anti-D sensitized red blood cells and determined the detection limit.
Main Results:
- The silk cocoon membrane-based immunosensor demonstrated potential for both qualitative and quantitative immunoassays.
- Colorimetric responses were observed across a concentration range of 1 μg/mL to 1 ng/mL, with a detection limit of 3.4 ng/mL for anti-D.
- The immunosensor exhibited excellent stability, with immobilized antibodies retaining bioactivity for up to 40 weeks at 4 and 25 °C.
- Bioactivity showed a decline after 12 weeks at 37 °C, indicating thermal stability within a certain range.
Conclusions:
- Natural silk cocoon membrane is a promising substrate for developing versatile and thermally stable immunosensing platforms.
- The developed immunosensor offers a sensitive and stable method for immunoassays, overcoming limitations of traditional materials.
- This approach highlights the potential of utilizing natural biomaterials in advanced diagnostic tools.
