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Pretreatment of Lignocellulosic Biomass with Low-cost Ionic Liquids
Published on: August 10, 2016
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Ionic liquid depolymerize the lignocellulose for the enzymatic extraction of feruloylated oligosaccharide from corn
Lingxiao Gong1, Dannin Feng1, Jie Liu1
1China-Canada Joint Lab of Food Nutrition and Health (Beijing), Beijing Engineering and Technology Research Center of Food Additives, School of Food and Health, Beijing Technology & Business University (BTBU), Beijing 100048, China.
Food Chemistry: X
|October 10, 2022
Summary
A novel method using ionic liquids (ILs) efficiently extracts feruloylated oligosaccharides (FOs) from corn bran. This approach significantly boosts FOs yield compared to traditional methods, offering a new route for valuable compound recovery.
Area of Science:
- Biotechnology
- Green Chemistry
- Food Science
Background:
- Dietary fiber valorization is crucial for sustainable resource utilization.
- Feruloylated oligosaccharides (FOs) are valuable compounds found in plant cell walls.
- Efficient extraction methods for FOs from agricultural byproducts like corn bran are needed.
Purpose of the Study:
- To develop and optimize an enzymatic hydrolysis extraction method for FOs from corn bran using ionic liquids (ILs).
- To investigate the effectiveness of different ILs and identify the optimal conditions for FOs extraction.
- To elucidate the mechanism of ILs in facilitating FOs release from lignocellulosic biomass.
Main Methods:
- Screening of eight ionic liquids (ILs) for their efficacy in depolymerizing corn bran dietary fiber.
- Optimization of extraction conditions (time, temperature, solid-liquid ratio, IL concentration) using response surface methodology.
- Characterization of the depolymerized biomass using Scanning Electron Microscopy (SEM), Fourier-Transform Infrared Spectroscopy (FTIR), and Confocal Laser Scanning Microscopy (CLSM).
Main Results:
- 1-allyl-3-methylimidazolium acetate ([Amim]Ac) was identified as the most effective IL, increasing total FOs content by 1.5 times compared to non-pretreatment methods.
- Optimal extraction conditions were determined as 194.31 min, 143.08 °C, a solid-liquid ratio of 1:20, and an [Amim]Ac concentration of 18.65%.
- SEM, FTIR, and CLSM analyses confirmed that [Amim]Ac facilitates FOs release by disrupting the lignocellulose structure and exposing hemicellulose binding sites, without affecting ferulic acid-hemicellulose linkages.
Conclusions:
- Ionic liquid pretreatment, particularly with [Amim]Ac, significantly enhances the enzymatic hydrolysis extraction of feruloylated oligosaccharides from corn bran.
- The optimized method provides an efficient and effective strategy for producing FOs and other conjugated phenols from lignocellulosic biomass.
- This study contributes to the sustainable valorization of agricultural residues by offering a green chemistry approach for extracting high-value compounds.
