Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

2.7K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.7K
Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

5.7K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
5.7K
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

7.4K
The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
7.4K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

2.3K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: Syndecan-2 selectively regulates VEGF-induced vascular permeability.

Nature cardiovascular research·2024
Same author

Second primary neoplasms in patients with lung and gastroenteropancreatic neuroendocrine neoplasms: Data from a retrospective multi-centric study.

Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·2021
Same author

The Reference Site Collaborative Network of the European Innovation Partnership on Active and Healthy Ageing.

Translational medicine @ UniSa·2019
Same author

The World Association of Radiopharmaceutical and Molecular Therapy position statement on the initial radioiodine therapy for differentiated thyroid carcinoma.

World journal of nuclear medicine·2019
Same author

Papillary Carcinoma in Mature Teratoma of Struma Ovarii.

Journal of the Belgian Society of Radiology·2018
Same author

Influence of peanut matrix on stability of allergens in gastric-simulated digesta: 2S albumins are main contributors to the IgE reactivity of short digestion-resistant peptides.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2018

Related Experiment Video

Updated: Aug 26, 2025

Author Spotlight: Investigating Angiogenesis and Vessel Permeability Through a Modified Matrix Gel Plug Assay
09:03

Author Spotlight: Investigating Angiogenesis and Vessel Permeability Through a Modified Matrix Gel Plug Assay

Published on: June 30, 2023

1.1K

Syndecan-2 selectively regulates VEGF-induced vascular permeability.

F Corti1, E Ristori1, F Rivera-Molina2

  • 1Yale Cardiovascular Research Center Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06511, USA.

Nature Cardiovascular Research
|October 10, 2022
PubMed
Summary

Syndecan-2 (Sdc2) regulates vascular permeability by controlling DEP1 phosphatase interaction with VEGFR2. Blocking Sdc2 reduces stroke size by decreasing brain edema.

More Related Videos

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors
09:04

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors

Published on: March 15, 2016

9.8K
Evaluating Vascular Hyperpermeability-inducing Agents in the Skin with the Miles Assay
08:43

Evaluating Vascular Hyperpermeability-inducing Agents in the Skin with the Miles Assay

Published on: June 19, 2018

15.1K

Related Experiment Videos

Last Updated: Aug 26, 2025

Author Spotlight: Investigating Angiogenesis and Vessel Permeability Through a Modified Matrix Gel Plug Assay
09:03

Author Spotlight: Investigating Angiogenesis and Vessel Permeability Through a Modified Matrix Gel Plug Assay

Published on: June 30, 2023

1.1K
A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors
09:04

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors

Published on: March 15, 2016

9.8K
Evaluating Vascular Hyperpermeability-inducing Agents in the Skin with the Miles Assay
08:43

Evaluating Vascular Hyperpermeability-inducing Agents in the Skin with the Miles Assay

Published on: June 19, 2018

15.1K

Area of Science:

  • Molecular biology
  • Cell biology
  • Physiology

Background:

  • Vascular endothelial growth factor (VEGF) increases vascular permeability, a key factor in inflammatory and ischemic diseases.
  • Specific regulators of VEGF-dependent permeability are not well understood.
  • VEGF plays crucial roles in various biological processes beyond vascular permeability.

Purpose of the Study:

  • To identify specific regulators of VEGF-driven vascular permeability.
  • To investigate the role of proteoglycan Syndecan-2 (Sdc2) in controlling VEGF receptor 2 (VEGFR2) signaling.
  • To explore the therapeutic potential of targeting Sdc2 in ischemic injury models.

Main Methods:

  • Investigated the interaction between Syndecan-2 (Sdc2), DEP1 phosphatase, and VEGFR2.
  • Utilized antibodies to block Sdc2-DEP1 interactions.
  • Examined the effect of Sdc2 manipulation on VEGFR2 phosphorylation at the Y951 site.
  • Assessed stroke size and intracerebral edema in an in vivo stroke model with endothelial-specific Sdc2 deletion or anti-Sdc2 antibody treatment.

Main Results:

  • Syndecan-2 (Sdc2) regulates the cell surface interaction between DEP1 and VEGFR2.
  • Absence of Sdc2 or blockade of Sdc2-DEP1 interaction leads to increased DEP1 at the plasma membrane.
  • Increased DEP1 promotes selective dephosphorylation of VEGFR2 at the Y951 site, crucial for permeability.
  • Endothelial-specific Sdc2 deletion or anti-Sdc2 antibody treatment significantly reduced stroke size and intracerebral edema.

Conclusions:

  • Syndecan-2 (Sdc2) acts as a specific regulator of VEGF-driven vascular permeability.
  • Targeting the Sdc2-DEP1 interaction offers a potential therapeutic strategy for reducing edema in ischemic stroke.
  • Modulating Sdc2 levels or function can selectively impact VEGF-mediated permeability without affecting other VEGFR2 functions.