Sequences that confer beta-tubulin autoregulation through modulated mRNA stability reside within exon 1 of a beta-tubulin mRNA
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View abstract on PubMed
Summary
This summary is machine-generated.Animal cells regulate alpha- and beta-tubulin synthesis via a novel autoregulatory mechanism. Unpolymerized tubulin subunits control tubulin mRNA levels, with specific mRNA sequences dictating this regulation.
Area Of Science
- Molecular Biology
- Cell Biology
- Genetics
Background
- Tubulin is essential for cell structure and function.
- Tubulin synthesis is tightly regulated in animal cells.
Purpose Of The Study
- To identify the specific sequences responsible for beta-tubulin mRNA autoregulation.
- To elucidate the mechanism by which tubulin subunit concentration controls tubulin mRNA levels.
Main Methods
- Transient DNA transfection assays.
- Construction of chimeric RNA molecules by inserting beta-tubulin gene sequences into a thymidine kinase gene.
- Analysis of chimeric RNA expression and regulation.
Main Results
- A short sequence (106 nucleotides) from the beta-tubulin gene, including 5' untranslated and coding regions, is sufficient to confer autoregulation.
- Deletion of 5' untranslated region sequences does not abolish regulation.
- Regulation is mediated by sequences within the first 16 translated codons.
Conclusions
- A novel autoregulatory pathway controls tubulin synthesis at the mRNA level.
- Cytoplasmic events modulating mRNA stability are key to this regulation.
- Specific sequences in the initial coding region of beta-tubulin mRNA are critical for autoregulation.
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