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Updated: Aug 26, 2025

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
06:02

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Characterization of Mayaro virus (strain BeAn343102) biology in vertebrate and invertebrate cellular backgrounds

Sujit Pujhari1,2, Marco Brustolin1,3, Chan C Heu1,4

  • 1Department of Entomology, Center for Infectious Disease Dynamics and the Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, USA.

The Journal of General Virology
|October 10, 2022
PubMed

Insights

Mayaro virus (MAYV) enters cells via pH-dependent endocytosis and macropinocytosis. This study details MAYV

Area of Science:

  • Virology
  • Cell Biology
  • Pathogenesis

Background:

  • Mayaro virus (MAYV) is an emerging alphavirus causing febrile illness, rash, and polyarthritis.
  • Understanding MAYV's lifecycle and cell pathology is crucial due to its expanding geographical presence.

Purpose of the Study:

  • To characterize Mayaro virus replication, cell entry, lifecycle, and cytopathic effects in vitro.
  • To elucidate the mechanisms of MAYV infection in both vertebrate and invertebrate cells.

Main Methods:

  • Utilized in vitro models of vertebrate and invertebrate cells.
  • Investigated viral entry using inhibitors and markers for endocytosis and macropinocytosis.
  • Examined replication complexes, virus release, and induction of apoptosis.

Main Results:

  • MAYV enters vertebrate cells through pH-dependent endocytosis and macropinocytosis.
  • Replication complexes form in the cytoplasm or associated with membranes in both cell types.
  • Virus release occurs via exocytosis and budding; MAYV induces apoptosis in vertebrate cells.

Conclusions:

  • MAYV employs multiple entry pathways into vertebrate cells.
  • The study provides foundational biological insights into MAYV infection.
  • Understanding MAYV's mechanisms is vital for developing therapeutic and preventive strategies.
Keywords:
Mayaro virusarboviruscytopathic vacuolesmacropinocytosisvirus–host interactions

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