Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HoloBio: A holographic microscopy tool for quantitative biological analysis.

PLoS computational biology·2026
Same author

Deoxygenation-free digital holographic microscopy discriminates sickle-cell disease in a piloted Colombian cohort via phase and shape metrics.

Optics express·2026
Same author

Enhanced red-light-driven hydrogen evolution by a diplatinum photocatalyst by the larger wavefunction leakage of iodide coordinated to the platinum center.

Chemical science·2025
Same author

Generalized computational framework for phase image reconstruction in structured illumination digital holographic microscopy.

Optics express·2025
Same author

Diplatinum Single-Molecular Photocatalyst Capable of Driving Hydrogen Production from Water via Singlet-to-Triplet Transitions.

Angewandte Chemie (International ed. in English)·2025
Same author

A 1:1 flavone cocrystal with cyclic trimeric perfluoro-<i>o</i>-phenyl-enemercury.

Acta crystallographica. Section E, Crystallographic communications·2024
Same journal

Invaders taking over-Mollusc faunal change in volcanic barrier lakes of the Albertine Rift biodiversity hotspot.

PloS one·2026
Same journal

AI-driven molecular diversification and ligand-based optimization of macitentan derivatives targeting VEGFR1 and endothelin signaling pathways.

PloS one·2026
Same journal

Performance patterns and records in the world aquatics masters championships: Where do the most frequently represented nations among the top-ten masters swimmers come from?

PloS one·2026
Same journal

Modeling diurnal Temperature-Rainfall relationships under multicollinearity using PLS-SEM: A case study of Ghana.

PloS one·2026
Same journal

Organizational culture, social capital, and emergency capacity in primary healthcare institutions: A cross-sectional structural equation modeling study comparing ordinary and older communities.

PloS one·2026
Same journal

Impact of kidney function on the metabolome in the general population.

PloS one·2026
See all related articles

Related Experiment Video

Updated: Aug 26, 2025

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

10.4K

pyDHM: A Python library for applications in digital holographic microscopy.

Raul Castañeda1, Carlos Trujillo2, Ana Doblas1

  • 1Optical Imaging Research Laboratory, Department of Electrical and Computer Engineering, The University of Memphis, Memphis, TN, United States of America.

Plos One
|October 10, 2022
PubMed
Summary
This summary is machine-generated.

pyDHM is an open-source Python library for Digital Holographic Microscopy (DHM). It offers algorithms for reconstructing amplitude and phase images in various DHM setups, validated with numerical and experimental data.

More Related Videos

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy DHM
07:27

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy DHM

Published on: November 1, 2017

10.4K
Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

12.3K

Related Experiment Videos

Last Updated: Aug 26, 2025

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

10.4K
Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy DHM
07:27

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy DHM

Published on: November 1, 2017

10.4K
Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

12.3K

Area of Science:

  • Optics and Photonics
  • Computational Imaging
  • Microscopy

Background:

  • Digital Holographic Microscopy (DHM) is a powerful technique for quantitative phase imaging.
  • Existing software solutions may lack flexibility or comprehensive algorithm support for diverse DHM configurations.
  • There is a need for accessible, open-source tools for DHM data processing.

Purpose of the Study:

  • To introduce pyDHM, an open-source Python library for Digital Holographic Microscopy (DHM).
  • To provide a user-friendly platform with diverse numerical algorithms for DHM image reconstruction.
  • To support various optical configurations and phase compensation techniques in DHM.

Main Methods:

  • Implementation of phase-shifting algorithms for in-line and off-axis DHM.
  • Inclusion of phase compensation for telecentric and non-telecentric optical systems.
  • Integration of three distinct propagation algorithms for numerical focusing in DHM and digital holography (DH).

Main Results:

  • pyDHM successfully reconstructs amplitude and phase images from DHM data.
  • The library supports a wide range of DHM setups, including phase-shifting and different optical geometries.
  • Validation using both numerical and experimental holograms confirms the library's accuracy and reliability.

Conclusions:

  • pyDHM offers a versatile and robust solution for DHM data analysis.
  • The open-source nature of pyDHM promotes accessibility and further development in the field.
  • This library empowers researchers to perform advanced quantitative phase imaging with Python.