Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Photoluminescence: Fluorescence and Phosphorescence01:23

Photoluminescence: Fluorescence and Phosphorescence

2.2K
Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
A pair of electrons in a...
2.2K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.1K
Photoluminescence: Applications01:14

Photoluminescence: Applications

473
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
473
Photoelectric Effect02:26

Photoelectric Effect

30.0K
When light of a particular wavelength strikes a metal surface, electrons are emitted. This is called the photoelectric effect. The minimum frequency of light that can cause such emission of electrons is called the threshold frequency, which is specific to the metal. Light with a frequency lower than the threshold frequency, even if it is of high intensity, cannot initiate the emission of electrons. However, when the frequency is higher than the threshold value, the number of electrons ejected...
30.0K
Variables Affecting Phosphorescence and Fluorescence01:26

Variables Affecting Phosphorescence and Fluorescence

577
Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...
577
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

699
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
699

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spectroscopic Characterization of the Charge-Separated Zwitterionic State Responsible for the Excimer-like Emission of a Luminescent Diradical.

Journal of the American Chemical Society·2025
Same author

Effects of trap sites on magnetic-field dependent electric conductance and recombination of carriers photogenerated in a dye-doped organic semiconductor film device.

The Journal of chemical physics·2025
Same author

A Fundamental Study on the Removal of Vascular Pulsation Artifacts Using U-Net-Based Deep Neural Network.

Cureus·2025
Same author

Small-field measurements in 3D polymer gel dosimetry using optical computed tomography.

Radiation protection dosimetry·2025
Same author

Artifact estimation network for MR images: effectiveness of batch normalization and dropout layers.

BMC medical imaging·2025
Same author

Triplet pair dynamics of singlet fission in orthorhombic polycrystalline powder of rubrene as revealed by magnetoluminescence.

The Journal of chemical physics·2025

Related Experiment Video

Updated: Aug 26, 2025

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation
11:20

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

Published on: August 30, 2017

7.5K

Photon Upconversion with a Low Threshold Excitation Intensity in Plain Water.

Yuki Nakadai1, Shuta Tsuchiya2, Masumi Uehara1

  • 1Department of Materials Science and Technology, Faculty of Engineering, Niigata University, 8050 Ikarashi-2, Niigata950-2181, Japan.

The Journal of Physical Chemistry. B
|October 10, 2022
PubMed
Summary

Researchers developed a novel photon upconversion system using triplet-triplet annihilation (TTA-UC) in plain water. This system achieves a low excitation intensity threshold, making it efficient for aqueous applications.

More Related Videos

Integrating a Triplet-triplet Annihilation Up-conversion System to Enhance Dye-sensitized Solar Cell Response to Sub-bandgap Light
11:26

Integrating a Triplet-triplet Annihilation Up-conversion System to Enhance Dye-sensitized Solar Cell Response to Sub-bandgap Light

Published on: September 12, 2014

12.7K
High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
10:40

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

7.6K

Related Experiment Videos

Last Updated: Aug 26, 2025

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation
11:20

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

Published on: August 30, 2017

7.5K
Integrating a Triplet-triplet Annihilation Up-conversion System to Enhance Dye-sensitized Solar Cell Response to Sub-bandgap Light
11:26

Integrating a Triplet-triplet Annihilation Up-conversion System to Enhance Dye-sensitized Solar Cell Response to Sub-bandgap Light

Published on: September 12, 2014

12.7K
High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
10:40

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

7.6K

Area of Science:

  • Photochemistry
  • Materials Science
  • Supramolecular Chemistry

Background:

  • Photon upconversion (UC) enhances light harvesting by converting lower-energy photons to higher-energy ones.
  • Triplet-triplet annihilation (TTA-UC) is a promising UC mechanism but often requires organic solvents and additives.
  • Developing efficient TTA-UC systems in aqueous media is crucial for biological and environmental applications.

Purpose of the Study:

  • To develop a TTA-UC system functional in plain water without surfactants.
  • To achieve a low threshold excitation intensity for aqueous TTA-UC.
  • To demonstrate the feasibility and efficiency of the developed aqueous TTA-UC system.

Main Methods:

  • Utilized water-soluble anionic porphyrin (PdTPPS4-) as a sensitizer and a diphenylanthracene derivative (DCDPA2-) as an emitter.
  • Investigated triplet energy transfer via phosphorescence quenching and fluorescence emission.
  • Employed three independent emission studies with different light sources to validate the TTA-UC process.
  • Measured time profiles of phosphorescence and fluorescence under pulse laser excitation to confirm TTA mechanism.

Main Results:

  • Successfully demonstrated TTA-UC in a simple aqueous solution using PdTPPS4- and DCDPA2-.
  • Observed efficient triplet energy transfer from PdTPPS4- to DCDPA2-, leading to DCDPA2- fluorescence emission.
  • Estimated a low threshold excitation intensity (Ith) for TTA-UC, below 6 mW cm-2.
  • Achieved an Ith comparable to high-performance TTA-UC systems in organic solutions, representing a new benchmark for aqueous systems.

Conclusions:

  • Developed the first TTA-UC system operating efficiently in plain water without additives.
  • The system exhibits a significantly low excitation intensity threshold, suitable for various aqueous applications.
  • This breakthrough paves the way for advanced photon upconversion technologies in biological imaging, sensing, and photocatalysis in aqueous environments.