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Updated: Aug 26, 2025

Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters
Published on: June 2, 2010
Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes
Xinzhu Xu1,2,3, Shu Jia2, Peng Xi4,5,6
1Department of Biomedical Engineering, College of Future Technology, Peking University, Beijing, 100871, China.
Abstract:
A donut excitation moves around a single molecule with a zigzag configuration lattice by lattice. Such a method implemented in scanning fluorescence microscopy simplifies the conventional MINFLUX process. Consisting of hollow zero-intensity excitation, single-pixel detection, time-correlated single photon counting, and drift stabilization, the system achieves localization precision and resolution very close to conventional MINFLUX theoretically and experimentally. An averaged high-SNR reference, and pixel-registered intensity from a single molecule is essential to reconstruct localization in maximum likelihood estimation. With performance reaching nearly conventional MINFLUX's, the proposed raster-scanning MINFLUX can inspire researchers expertized in STED or confocal setup to quickly transform to MINFLUX and develop for further exploring on bio-specimens or optical applications.
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