Nanoenzyme-chitosan hydrogel complex with cascade catalytic and self-reinforced antibacterial performance for
Zhiguo Li1, Xiaotong Fan2, Zheng Luo1,3
1Fujian Provincial Key Laboratory of Innovative Drug Target Research and State Key Laboratory of Cellular Stress Biology, School of Pharmaceutical Sciences, Xiamen University, Xiamen 361102, China. wuyl@xmu.edu.cn.
Abstract:
The significant disability and fatality rate of diabetes chronic wounds necessitates the development of efficient diabetic wound healing techniques. The present oxygen treatments for wound healing is restricted by issues such as poor penetration, inadequate supply, and absorption difficulties as well as tanglesome diabetic wound microenvironment issues such as hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia. Herein, we designed a multifunctional glucose oxidase (GOx) and catalase (CAT) nanoenzyme-chitosan (GCNC) hydrogel complex to improve the microenvironment of diabetic wounds and provide continuous oxygen delivery for efficient wound healing. By simultaneously forming the GOx-CAT nanoenzyme (GCNE) composite, the GCNC hydrogel complex could effectively reduce glucose and ROS (H2O2) concentrations in diabetic wounds through cascade catalytic reactions and achieve continuous oxygen supply, which promoted cell proliferation, migration, and angiogenesis, thereby accelerating diabetic wound healing. In addition, the byproduct gluconic acid produced by the cascade reaction can activate the amino group of chitosan to reinforce the antibacterial performance and prevent microbial infection. This multifunctional GCNC hydrogel complex with continuous oxygen supply, self-reinforcing antibacterial properties, and byproduct-free features provides a general strategy for repairing the extensive tissue damage in diabetes.
Insights
This study introduces a novel glucose oxidase and catalase nanoenzyme-chitosan hydrogel complex to enhance diabetic wound healing. The GCNC hydrogel complex effectively manages the wound microenvironment, promoting healing and reducing infection risks.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Wound Healing
Background:
- Diabetic chronic wounds present significant healing challenges due to impaired microenvironments, including hyperglycemia, excessive reactive oxygen species (ROS), and hypoxia.
- Current oxygen-based therapies for wound healing are limited by poor penetration, inadequate supply, and absorption issues.
Purpose of the Study:
- To design and evaluate a multifunctional glucose oxidase (GOx) and catalase (CAT) nanoenzyme-chitosan (GCNC) hydrogel complex for improved diabetic wound healing.
- To address the limitations of existing treatments by creating a system that manages the diabetic wound microenvironment and provides continuous oxygen.
Main Methods:
- Fabrication of a GCNC hydrogel complex incorporating a GOx-CAT nanoenzyme (GCNE) composite.
- Evaluation of the hydrogel's ability to reduce glucose and ROS (H2O2) concentrations via cascade catalytic reactions.
- Assessment of continuous oxygen supply and its impact on cell proliferation, migration, and angiogenesis in a diabetic wound model.
Main Results:
- The GCNC hydrogel complex effectively reduced glucose and ROS levels in diabetic wound models.
- Continuous oxygen supply from the hydrogel promoted cell proliferation, migration, and angiogenesis.
- The byproduct gluconic acid activated chitosan, enhancing antibacterial properties and preventing infection.
Conclusions:
- The multifunctional GCNC hydrogel complex offers a promising strategy for accelerating diabetic wound healing.
- This approach effectively improves the diabetic wound microenvironment, provides sustained oxygen, and possesses self-reinforcing antibacterial properties.
- The byproduct-free nature and enhanced functionalities make GCNC hydrogels a versatile platform for treating extensive diabetic tissue damage.
