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Interference of morintides on some virulence determinants of selected bacterial pathogens

Qudsia Jaffar1, Uzma Qaisar2, Muhammad Shahid1

  • 1Department of Biochemistry, University of Agriculture Faisalabad, Pakistan.

Pakistan Journal of Pharmaceutical Sciences
|October 11, 2022
PubMed

Insights

Antimicrobial peptides from Moringa oleifera L, called morintides, show potential in combating bacterial virulence factors like biofilms and siderophores. This research contributes to developing new drugs against antibiotic-resistant bacteria.

Area of Science:

  • Microbiology
  • Biochemistry
  • Pharmacology

Background:

  • Antimicrobial peptides (AMPs) are crucial in innate immunity.
  • Moringa oleifera L. is a plant with known medicinal properties.
  • Bacterial biofilms and siderophores are key virulence factors contributing to antibiotic resistance.

Purpose of the Study:

  • To isolate and partially purify antimicrobial peptides (AMPs) from Moringa oleifera L. (morintides).
  • To investigate the potency of morintides in interfering with bacterial virulence determinants, including biofilms, siderophores, and elastase.
  • To evaluate the efficacy of morintides against selected bacterial strains, including antibiotic-resistant ones.

Main Methods:

  • Ultra-15 Centrifugal filter devices for AMP separation.
  • Spectrophotometric methods and Thin-layer chromatography (TLC) for virulence factor analysis.
  • Statistical analysis using GraphPad Prism 5.0 with two-way ANOVA and Bonferroni post-test.

Main Results:

  • Morintides reduced biofilm formation in Shigella flexneri, Klebsiella pneumoniae, Escherichia coli, and Staphylococcus aureus, but increased it in Pseudomonas aeruginosa and Salmonella typhi.
  • Morintides effectively disrupted developed biofilms in S. aureus, E. coli, K. pneumoniae, and S. flexneri.
  • Siderophore production was decreased in most tested bacterial strains, except P. aeruginosa.

Conclusions:

  • Morintides exhibit significant antimicrobial activity against key bacterial virulence factors.
  • These findings suggest morintides as potential candidates for novel drug discovery against antibiotic-resistant bacterial infections.
  • Further research is warranted to explore the therapeutic potential of morintides in combating biofilm-associated infections.

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