Related Experiment Video

Updated: Aug 26, 2025

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
06:01

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization

Published on: June 7, 2016

6.8K

Integrative Expression, Survival Analysis and Cellular miR-2909 Molecular Interplay in MRN Complex Check Point Sensor

Jitender Singh1, Namrata Sangwan1, Arushi Chauhan1

  • 1Department of Biophysics, Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh, India, 160012.

Clinical Breast Cancer
|October 11, 2022
PubMed
Abstract

Insights

MicroRNA-2909 (miR-2909) shows a potential role in breast cancer by forming stable complexes with DNA repair genes MRE11 and RAD50. This interaction, involving argonaute protein, suggests miR-2909 as a molecular marker for breast cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Breast cancer is a significant global health concern.
  • MicroRNAs (miRNAs) are increasingly recognized for their role in DNA repair gene variants, including MRE11, RAD50, and NBN (collectively MRN-CSG).
  • The specific interactions between MRN-CSG mutations and miRNAs, particularly miR-2909, in breast cancer remain largely unexplored.

Purpose of the Study:

  • To investigate the role of miR-2909 as a molecular mechanistic marker in breast cancer.
  • To analyze genomic and proteomic data of MRN-CSG in breast cancer patients to identify mutation types and their association with survival rates.
  • To perform molecular, 3D-structural, and functional analyses to elucidate miR-2909's regulatory role in MRN-CSG.

Main Methods:

  • Genomic and proteomic data analysis of MRN-CSG in 8426 breast cancer patients.
  • Kaplan-Meier survival curve analysis to correlate mutations with survival rates.
  • Network analysis, disease association studies, molecular hybridization, and argonaute protein docking to assess miR-2909 interactions with MRN-CSG.

Main Results:

  • Genetic alterations in MRN-CSG were identified, predominantly missense mutations, with MRE11 showing higher mRNA expression in invasive ductal breast carcinoma.
  • Patients with unaltered MRN-CSG groups exhibited higher survival rates compared to altered groups.
  • miR-2909 demonstrated strong interactions with MRN-CSG, forming thermodynamically stable hybridized complexes with MRE11 and RAD50 mRNAs, and exhibiting strong binding affinity with argonaute protein.

Conclusions:

  • miR-2909 plays a significant molecular mechanistic role in breast cancer.
  • The formation of stable molecular complexes between miR-2909 and MRE11/RAD50 mRNAs, along with argonaute protein interaction, highlights its potential as a biomarker.
  • Further research into miR-2909's function could lead to novel therapeutic strategies for breast cancer.
Keywords:
Breast cancerGibbs free energy and hybridizationMRN complexNetworkmiR-2909

More Related Videos

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.7K
Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells16:24

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

20.3K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.9K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
3.8K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K