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First Isolation of Vancomycin-Resistant Enterococcus faecium Carrying Plasmid-Borne vanD1
Junzo Hisatsune1,2,3, Koichi Tanimoto4, Tadahiro Kohara3,5
1Antimicrobial Resistance Research Center, National Institute of Infectious Diseasesgrid.410795.e, Higashimurayama, Tokyo, Japan.
Abstract:
Vancomycin-resistant Enterococcus faecium carrying the vanD1 gene on plasmid pEF-D was isolated from a fecal sample of a hospitalized patient in Japan. The strain JH5687 showed moderate resistance to vancomycin (MIC, 16 μg/mL) but remained susceptible to teicoplanin (MIC, 1 μg/mL). The backbone gene organization of pEF-D was highly homologous to that of conjugative plasmid pMG1 or pHTβ. The calculated conjugation frequency of JH5687 was 10-4 to 10-5 per donor cell.
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