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Updated: Aug 26, 2025
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Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
Published on: June 11, 2015
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Complete Genome Sequence of Pseudomonas Phage Motto
Prasanth Manohar1, Archana Loganathan2, Ramesh Nachimuthu2
1Zhejiang University-University of Edinburgh (ZJE-UoE) Institute, Zhejiang University School of Medicine, Haining, People's Republic of China.
Microbiology Resource Announcements
|October 12, 2022
Summary
We sequenced the genome of bacteriophage Motto, which infects Pseudomonas aeruginosa. This T1-like siphovirus provides insights into phage-genetics and potential therapeutic applications.
Area of Science:
- Microbiology
- Virology
- Genomics
Background:
- Pseudomonas aeruginosa is an opportunistic pathogen causing significant infections.
- Bacteriophages are viruses that infect bacteria and are being explored for phage therapy.
- Understanding phage-bacterial interactions is crucial for developing novel antimicrobial strategies.
Purpose of the Study:
- To present the complete genome sequence of bacteriophage Motto.
- To characterize Motto's genomic features and taxonomic placement.
- To provide a resource for studying Pseudomonas aeruginosa phages.
Main Methods:
- Whole-genome sequencing of bacteriophage Motto.
- Bioinformatic analysis of the genome sequence.
- Comparative genomics to determine taxonomic relationships.
Main Results:
- The complete genome sequence of bacteriophage Motto (49.9 kb) was determined.
- Motto was identified as a T1-like siphovirus, related to the Drexlerviridae family.
- The genome encodes 84 protein-coding genes, with specific morphological characteristics (57 nm capsid, 255 nm tail).
Conclusions:
- The genome sequence of bacteriophage Motto offers valuable data for Pseudomonas aeruginosa research.
- Motto's classification within T1-like siphoviruses aids in understanding phage evolution.
- This genomic information can contribute to the development of phage-based interventions against P. aeruginosa infections.
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