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Rapid, Safe, and Simple Manual Bedside Nucleic Acid Extraction for the Detection of Virus in Whole Blood Samples

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Monkeypox Virus Detection in Different Clinical Specimen Types.

Maan Hasso, Stephen Perusini, Alireza Eshaghi

Emerging Infectious Diseases

|October 12, 2022

Summary

This summary is machine-generated.

The 2022 monkeypox outbreak highlighted the need for effective diagnostics. Clinical evaluation revealed that skin lesion samples are the best source for accurate monkeypox molecular testing.

Keywords:

Canada MPXV clades clinical diagnostics clinical specimen types monkeypox monkeypox virus preferred specimen source viruses zoonoses

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Published on: June 30, 2018

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Area of Science:

Virology
Infectious Diseases
Molecular Diagnostics

Background:

A global monkeypox outbreak emerged in May 2022, necessitating rapid diagnostic strategies.
Limited data were available regarding the performance of various specimen types in monkeypox molecular diagnostics during the early outbreak phase.

Purpose of the Study:

To evaluate the performance of different specimen types for monkeypox molecular diagnostics.
To identify the optimal specimen source for accurate diagnosis during the 2022 outbreak.

Main Methods:

Collection of diverse specimen types from suspected monkeypox cases in Ontario, Canada, during the initial weeks of the outbreak.
Clinical evaluation of specimen performance in associated molecular diagnostic assays.

Main Results:

Skin lesions were identified as the optimal diagnostic specimen source.
Performance data for other specimen types were also gathered, contributing to diagnostic understanding.

Conclusions:

Skin lesion sampling is recommended for accurate monkeypox molecular diagnosis.
This finding aids in optimizing diagnostic workflows during public health emergencies.