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Updated: Aug 26, 2025

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

Exploring vast microbial epitope spaces.

Marc Marius Geissler¹, Sebastian Zeissig¹

¹Center for Regenerative Therapies, Technische Universität (TU) Dresden, 01307 Dresden, Germany; Department of Medicine I, University Medical Center Dresden, Technische Universität (TU) Dresden, 01307 Dresden, Germany.

|October 12, 2022

Summary

This summary is machine-generated.

This study systematically analyzes bacterial HLA II epitopes and their corresponding CD4+ T cell responses, revealing plasticity in these interactions. Understanding these bacterial epitopes is crucial for immune system research.

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Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

Area of Science:

Immunology
Microbiome research
T cell immunology

Background:

The specific HLA epitopes presented by commensal bacteria remain largely uncharacterized.
The origin and diversity of these bacterial epitopes are not well understood.
The impact of bacterial HLA epitopes on host immune responses requires further investigation.

Purpose of the Study:

To systematically analyze the spectrum and origin of commensal bacterial HLA II epitopes.
To investigate the plasticity of CD4+ T cell responses to these bacterial epitopes.
To enhance the understanding of host-microbiome interactions at the epitope level.

Main Methods:

Bioinformatic analysis of bacterial proteomes to predict HLA II epitopes.
In vitro assays to characterize T cell recognition of predicted epitopes.
Analysis of T cell receptor repertoire associated with bacterial epitopes.

Main Results:

Identification of a diverse repertoire of commensal bacterial HLA II epitopes.
Demonstration of plasticity in CD4+ T cell responses, with varying epitope recognition.
Evidence for cross-reactivity between bacterial and self-epitopes in some cases.

Conclusions:

Commensal bacteria present a broad range of HLA II epitopes that can elicit CD4+ T cell responses.
The plasticity of T cell responses suggests dynamic interactions between the microbiome and the host immune system.
Further research into bacterial epitopes is essential for understanding immune homeostasis and disease.