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Jon G Sanders1, Weiwei Yan2, Deus Mjungu3

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Researchers developed a cost-effective bacterial whole-genome sequencing workflow. This method analyzes genomic diversity in gut microbes from primates, enabling new population genetic insights into bacterial strains.

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Area of Science:

  • Microbiology
  • Genomics
  • Evolutionary Biology

Background:

  • Microbial communities are crucial for Earth's processes, yet their evolution and function are poorly understood.
  • Current methods for studying microbial genomes are often low-throughput and expensive, limiting comprehensive analysis.

Purpose of the Study:

  • To develop a high-throughput, low-cost workflow for bacterial whole-genome sequencing.
  • To investigate genomic diversity within gut bacterial species of wild chimpanzees and bonobos.
  • To enable population genetic analyses of bacterial strains.

Main Methods:

  • Utilized open-source labware and the OpenTrons robotics platform for automated sample preparation.
  • Performed bacterial whole-genome sequencing on 45 gut bacterial species.
  • Quantified intraspecific genomic diversity and analyzed plasmid divergence.

Main Results:

  • Achieved a cost of approximately $10 per genome using the developed workflow.
  • Revealed significant genomic diversity within the studied gut bacterial species.
  • Identified divergence of homologous plasmids between chimpanzee and bonobo hosts.

Conclusions:

  • The presented workflow significantly reduces the cost and increases the throughput of bacterial whole-genome sequencing.
  • This approach provides a powerful tool for population genetic studies of microbial communities.
  • The findings offer new insights into the evolution and diversity of primate gut microbiomes.