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Updated: Aug 26, 2025

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
Ozone-3,6-dihydroxynaphtha-2,7-disulphonate chemiluminescence system is used for online ozone detection
Yalin Xiong1, Zhuqing Wang2, Lianbo Tang1
1School of Chemical Engineering, Sichuan University of Science & Engineering, 180 Xueyuan Street, Huixing Road, Zigong City, China.
Abstract:
The chemiluminescence (CL) reaction between ozone and 3,6-dihydroxynaphtha-2,7-disulphonate (DNDS) was found under alkaline conditions. Therefore, a novel CL system for ozone detection was established. The CL signal of the CL system is weak, and the CL signal is enhanced by adding nonionic surfactants. It was found that adding 16.4 g/l Triton X-100 can enhance the CL signal. The CL reagent activated by ultraviolet (UV) light produced a CL signal was nearly 10 times stronger than the CL reagent not activated by UV light; the CL signal was enhanced by adding 8 g/l NaHCO3 to the CL reagent irradiated by UV light. Through the optimization of these test conditions, a high-selectivity, high-sensitivity online detection method for ozone CL was established. The linear range was 0.5-150 ppbv, and the limit of detection (LOD) was 0.092 ppbv (S/N = 3).
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