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Updated: Aug 26, 2025

Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane
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SLC18A3 promoted renal cancer development through acetylcholine/cAMP signaling

Peng Tie1, Ji Cheng1, Miao-Xin Xue1

  • 1Department of Urology, Shaanxi Provincial People's Hospital Xi'an, Shaanxi Province, China.

American Journal of Cancer Research
|October 13, 2022
PubMed

Insights

This study reveals that the vesicular acetylcholine transporter (SLC18A3) promotes renal cancer growth and metastasis by enhancing acetylcholine signaling. Targeting SLC18A3 may offer new treatment strategies for this aggressive cancer.

Area of Science:

  • Oncology
  • Neuroscience
  • Molecular Biology

Background:

  • Renal cancer is highly metastatic and chemotherapy-resistant.
  • Key drivers of renal cancer development are not fully understood.
  • Acetylcholine (ACh) signaling is implicated in cancer progression.

Purpose of the Study:

  • To investigate the role of acetylcholine signaling in renal cancer.
  • To identify novel molecular targets for renal cancer treatment.

Main Methods:

  • Identified SLC18A3 expression in renal cancer patients.
  • Investigated the effect of SLC18A3 on ACh uptake and downstream signaling.
  • Utilized a mouse model (A489-bearing mice) to assess tumor growth and metastasis.

Main Results:

  • SLC18A3 was upregulated in renal cancer patients.
  • SLC18A3 enhanced ACh uptake, activating the PKA/CREB pathway.
  • SLC18A3 overexpression promoted tumor growth and lung metastasis in mice.

Conclusions:

  • SLC18A3 promotes renal cancer progression and metastasis via the ACh/PKA/CREB pathway.
  • SLC18A3 represents a potential therapeutic target for renal cancer.
  • Findings may guide the development of novel anti-cancer treatments.
Keywords:
CREBPKASLC18A3acetylcholinerenal cancer

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