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Updated: Aug 26, 2025

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
Published on: October 18, 2022
Design of Gene Boolean Gates and Circuits with Convergent Promoters
Biruck Woldai Abraha1, Mario Andrea Marchisio2
1School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China.
Abstract:
Gene digital circuits are the subject of many research works due to their various potential applications, from hazard detection to medical diagnostic. Moreover, a remarkable number of techniques, developed in electronics, can be used for the construction of biological digital systems. In our previous works, we showed how to automatize the design and modeling of gene digital circuits whose gates were based on transcription and translation regulation. In this chapter, we illustrate how Boolean gates could be implemented by following a particular architecture, the convergent promoter one, rather diffuse in nature but seldom adopted in Synthetic Biology. Beside gate design, we also explain how to extend our previous modeling approach, based on composable parts and pools of molecules, to quantitatively describe and simulate this particular kind of digital biological devices.
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