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Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
Published on: February 21, 2018
FGFR2-CCDC6 fusion gene promotes the proliferation of Hucct-1 cells
Xianyan Wang1, Hongmei Li2, Ning Zhang1
1Department of Basic Pathology, School of Pathology, Qiqihar Medical University, Qiqihar, Heilongjiang161006, China. f.rahimi191@gmail.com.
Abstract:
To investigate the effect of the FGFR2-CCDC6 fusion gene on cell proliferation and its mechanism of action, pCDNA3.1- FGFR2bWT, pCDNA3.1- FGFR2-CCDC6 expression plasmids were transiently transfected into Hucct-1 cells using Lipo-2000 liposomes. The effect of the fusion gene on cell proliferation was examined by MTT and the expression of FGFR2/AKT/signaling pathway proteins was detected by Western blot. Results showed that Hucct-1 cells transfected with the FGFR2-CCDC6 fusion gene showed increased FGFR2 protein expression (P<0.001) and significantly higher cell proliferation capacity (P<0.001) compared to normal controls. It was concluded that The FGFR2-CCDC6 fusion gene excessively activates the AKT, and ERK signaling pathway downstream of FGFR2 and plays a role in promoting cell proliferation.
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