Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

7.8K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
7.8K
T Cell Types and Functions01:24

T Cell Types and Functions

1.3K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Head-to-head comparison of nuclear imaging approaches to quantify tumor CD8<sup>+</sup> T-cell infiltration.

Immunotherapy advancesĀ·2026
Same author

Sialic acid <i>cis</i>-ligand dynamics modulate Siglec-7 and -9 function and affect Siglec-7/9 co-blockade to potentiate natural killer cell anti-tumor activity.

OncoimmunologyĀ·2026
Same author

In-vivo analysis of neuroblastoma targeting potential of aGD2-SIRPĪ± fusion antibodies for local CD47 blockade.

Molecular cancer therapeuticsĀ·2026
Same author

Overcoming CXCR4-Mediated T-Cell Exclusion Potentiates Antitumor Cytotoxicity in Fibrolamellar Carcinoma.

GastroenterologyĀ·2026
Same author

Head and Neck Squamous Cell Carcinoma Patient-Derived Cancer-Associated Fibroblasts Undergo Senescence While Retaining Protumorigenic Properties After Ex Vivo Irradiation.

International journal of radiation oncology, biology, physicsĀ·2026
Same author

The radiotherapy induced cyclic GMP-AMP-synthase re-localization and immune signature predicts HNSCC treatment efficacy.

Communications medicineĀ·2026

Related Experiment Video

Updated: Aug 25, 2025

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro
16:13

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro

Published on: June 13, 2011

20.1K

NSAIDs affect dendritic cell cytokine production.

Tonke K Raaijmakers1,2, Renske J E van den Bijgaart1, Gert Jan Scheffer2

  • 1Department of Radiation Oncology, Radiotherapy & OncoImmunology Laboratory, Radboud Institute for Molecular Life Sciences, Radboud UMC, Nijmegen, The Netherlands.

Plos One
|October 13, 2022
PubMed
Summary

Non-steroid anti-inflammatory drugs (NSAIDs) can impair dendritic cell (DC) function, potentially affecting cancer immunotherapy. This study investigated NSAID impacts on DC cross-presentation, co-stimulation, and cytokine production for anti-tumor immunity.

More Related Videos

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1&#946; in Human Monocyte-derived Dendritic Cells
09:04

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Published on: May 22, 2014

21.0K
Teasing Out the Interplay Between Natural Killer Cells and Nociceptor Neurons
09:40

Teasing Out the Interplay Between Natural Killer Cells and Nociceptor Neurons

Published on: June 30, 2022

2.3K

Related Experiment Videos

Last Updated: Aug 25, 2025

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro
16:13

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro

Published on: June 13, 2011

20.1K
Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1&#946; in Human Monocyte-derived Dendritic Cells
09:04

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Published on: May 22, 2014

21.0K
Teasing Out the Interplay Between Natural Killer Cells and Nociceptor Neurons
09:40

Teasing Out the Interplay Between Natural Killer Cells and Nociceptor Neurons

Published on: June 30, 2022

2.3K

Area of Science:

  • Immunology
  • Cancer Research
  • Pharmacology

Background:

  • Immunotherapy is a cornerstone of cancer treatment, with dendritic cells (DCs) crucial for initiating anti-tumor immune responses.
  • DCs present tumor antigens to CD8+ T cells, a key step in adaptive immunity.
  • The impact of commonly used non-steroid anti-inflammatory drugs (NSAIDs) on DC function remains largely unexplored.

Purpose of the Study:

  • To investigate the effects of NSAIDs (diclofenac, ibuprofen, celecoxib) on critical DC functions.
  • To assess NSAID influence on antigen cross-presentation, co-stimulatory molecule expression, and cytokine production.
  • To understand how NSAIDs might impact T cell priming and the efficacy of cancer immunotherapy.

Main Methods:

  • Human monocyte-derived and murine bone-marrow derived DCs were utilized.
  • DCs were exposed to diclofenac, ibuprofen, and celecoxib.
  • Key DC functions including antigen cross-presentation, co-stimulatory marker expression, and cytokine production were analyzed.

Main Results:

  • NSAID exposure diminished Toll-like receptor (TLR)-induced pro-inflammatory cytokine excretion by DCs.
  • NSAID exposure also reduced anti-inflammatory cytokine excretion by DCs.
  • These effects were observed in both human and murine DC models.

Conclusions:

  • Various NSAIDs can significantly affect dendritic cell function.
  • NSAID-induced alterations in DC function may compromise their ability to prime T cells.
  • Further research is warranted to determine the clinical implications of NSAID use on cancer immunotherapy effectiveness.