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Extracellular vesicles derived from Pinctada martensii mucus regulate skin inflammation via the NF-κB/NLRP3/MAPK

Zijie Wu1, Lihua Ma1, Peichun Lin2

  • 1College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, 524088, China.

Biochemical and Biophysical Research Communications
|October 13, 2022
PubMed

Insights

Marine shellfish mucus yields novel extracellular vesicles (EVs) that reduce skin inflammation by inhibiting key inflammatory pathways and reducing cellular damage. These findings highlight potential new therapeutic applications for shellfish-derived bioactive materials.

Area of Science:

  • Biotechnology
  • Marine Biology
  • Immunology

Background:

  • Extracellular vesicles (EVs) are nanoparticles secreted by cells, containing bioactive molecules with therapeutic potential.
  • Research on extracting EVs from marine sources, particularly shellfish, is limited.
  • Pinctada martensii mucus is a potential source of novel bioactive materials.

Purpose of the Study:

  • To isolate extracellular vesicles (EVs) from Pinctada martensii mucus.
  • To investigate the anti-inflammatory efficacy of these mucus-derived EVs.
  • To elucidate the underlying molecular mechanisms of their anti-inflammatory action.

Main Methods:

  • Isolation of EVs from Pinctada martensii mucus.
  • Establishment of a human skin inflammatory cell model (HaCaT cells).
  • Assessment of cell viability, reactive oxygen species (ROS) levels, and inflammatory gene (IL-6, IL-8, TNF-α) expression.
  • Analysis of signaling pathways including MAPK, NF-κB, and NLRP3 inflammasome.

Main Results:

  • Pinctada martensii mucus-derived EVs restored the viability of inflammatory HaCaT cells.
  • EVs significantly decreased reactive oxygen species (ROS) levels and mRNA expression of IL-6, IL-8, and TNF-α.
  • EVs inhibited inflammation by suppressing MAPK, NF-κB, and NLRP3 inflammasome pathways, reducing inflammatory protein phosphorylation and P65 nuclear translocation.

Conclusions:

  • Extracellular vesicles derived from Pinctada martensii mucus possess significant anti-inflammatory properties.
  • These EVs modulate inflammatory responses through the inhibition of critical signaling pathways.
  • This study introduces a novel source of therapeutic EVs from marine shellfish bioactive materials.
Keywords:
Extracellular vesicleInflammationMAPKNF-κBNLRP3Pinctada martensii mucus

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