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Corynoxine suppresses pancreatic cancer growth primarily via ROS-p38 mediated cytostatic effects

Chunmei Wen1, Qingqing Ruan1, Zhaofeng Li2

  • 1Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, 325000, Wenzhou, China.

British Journal of Cancer
|October 13, 2022
PubMed
Abstract

Insights

Corynoxine (Cory) demonstrates anti-tumour effects against pancreatic cancer by inhibiting cell proliferation and inducing apoptosis. This compound shows promise as a potential therapeutic agent for pancreatic cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Pancreatic cancer is a leading cause of cancer mortality with limited effective treatments.
  • Corynoxine (Cory), known to induce autophagy in neuronal cells, has not been investigated for its anti-cancer potential in pancreatic cancer.

Purpose of the Study:

  • To investigate the anti-tumour activities of Corynoxine (Cory) against pancreatic cancer cell lines.
  • To elucidate the underlying mechanisms of Cory's anti-cancer effects.

Main Methods:

  • Utilized human pancreatic cancer cell lines (Patu-8988, Panc-1) for in vitro studies.
  • Assessed cell viability, proliferation, apoptosis, and colony formation.
  • Analyzed mRNA and protein expression via qPCR and Western blot.
  • Evaluated in vivo anti-tumour efficacy using a xenograft mouse model.

Main Results:

  • Cory treatment significantly inhibited pancreatic cancer cell proliferation and induced apoptosis.
  • Cory induced endoplasmic reticulum (ER) stress and reactive oxygen species (ROS) production.
  • Inhibition of ER stress partially rescued Cory-induced apoptosis, while ROS scavenging and p38 pathway inhibition reduced Cory's anti-proliferative effects.
  • Cory demonstrated significant tumour growth inhibition in vivo.

Conclusions:

  • Corynoxine exhibits anti-tumour effects in pancreatic cancer, primarily through ROS-p38 mediated cytostatic mechanisms.
  • Cory may represent a promising therapeutic candidate for pancreatic cancer.

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