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Identification and Characterization of lncRNAs Expression Profile Related to Goat Skeletal Muscle at Different
Haiyin Han1, Xianwei Wang2, Wentao Li1
1School of Life Sciences and Food Engineering, Hebei University of Engineering, Handan 056021, China.
Animals : an Open Access Journal From MDPI
|October 14, 2022
Summary
This study reveals long non-coding RNA (lncRNA) expression in goat skeletal muscle, identifying differentially expressed lncRNAs and potential regulatory networks. Findings enhance understanding of lncRNA roles in muscle development.
Area of Science:
- Genomics
- Molecular Biology
- Animal Science
Background:
- Long non-coding RNAs (lncRNAs) are crucial regulators of skeletal muscle development.
- The expression profile and functional roles of lncRNAs in goat muscle remain largely uncharacterized.
Purpose of the Study:
- To investigate the lncRNA expression profile in Wu'an black goat longissimus dorsi muscle.
- To identify differentially expressed lncRNAs (DE lncRNAs) between different age groups.
- To construct regulatory networks involving lncRNAs, miRNAs, and mRNAs to understand skeletal muscle development.
Main Methods:
- RNA sequencing of longissimus dorsi tissues from 1-month-old and 9-month-old Wu'an black goats.
- Bioinformatic analysis to identify and classify lncRNAs, and detect differentially expressed lncRNAs.
- RT-qPCR validation of selected lncRNAs.
- Construction of lncRNA-miRNA-mRNA interaction networks.
Main Results:
- A total of 3441 lncRNAs were identified, including lincRNAs, antisense lncRNAs, and sense_overlapping lncRNAs.
- 36 differentially expressed lncRNAs (DE lncRNAs) were identified between the age groups.
- DE lncRNAs were found to be neighboring protein-coding genes enriched in muscle development pathways.
- A lncRNA-miRNA-mRNA network was constructed, suggesting XR_001296113.2 may regulate PDLIM7 expression.
Conclusions:
- This study provides a comprehensive catalog of lncRNAs in goat skeletal muscle.
- Identified DE lncRNAs and regulatory networks offer insights into the molecular mechanisms of goat muscle development.
- The findings lay the groundwork for future research on lncRNA functions in livestock muscle growth and quality.
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