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PRELP Regulates Cell-Cell Adhesion and EMT and Inhibits Retinoblastoma Progression

Jack Hopkins1, Ken Asada2,3, Alex Leung1

  • 1UCL Institute of Ophthalmology, University College London, London EC1V 9EL, UK.

Cancers
|October 14, 2022
PubMed

Insights

PRELP acts as a tumor suppressor in retinoblastoma (RB), a common pediatric eye cancer. Loss of PRELP promotes cancer progression by reducing cell adhesion and increasing EMT, suggesting PRELP as a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Retinoblastoma (RB) is the most common intraocular pediatric cancer, often linked to mutations in the RB1 tumor suppressor gene.
  • PRELP, previously identified as a tumor suppressor regulating EMT, is located near a chromosomal region associated with RB initiation.
  • PRELP expression is downregulated in various cancers, indicating its potential role in tumorigenesis.

Purpose of the Study:

  • To investigate the role of the PRELP gene in the development and progression of retinoblastoma.
  • To explore PRELP's function as a tumor suppressor within the context of RB.
  • To identify potential therapeutic strategies targeting PRELP for RB treatment.

Main Methods:

  • In vitro analysis of RB cells.
  • Next-generation sequencing.
  • Immunostaining to detect PRELP expression in retinal Müller glial cells.
  • mRNA expression profiling in mouse retina and RB cells.

Main Results:

  • PRELP is expressed in Müller glial cells in the retina.
  • Loss of PRELP in RB cells reduces cell-cell adhesion.
  • Downregulation of PRELP facilitates epithelial-mesenchymal transition (EMT) in RB.
  • PRELP influences the cancer microenvironment, impacting RB progression.

Conclusions:

  • PRELP functions as a tumor suppressor in retinoblastoma.
  • PRELP's regulation of cell adhesion and EMT is crucial in RB progression.
  • PRELP represents a potential novel therapeutic target for retinoblastoma treatment.
Keywords:
EMT/METPRELPcell–cell adhesiondysplasiaretinoblastoma

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