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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Related Experiment Video

Updated: Aug 25, 2025

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
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Technologies Enabling Single-Molecule Super-Resolution Imaging of mRNA.

Mark Tingey1, Steven J Schnell1, Wenlan Yu1

  • 1Department of Biology, Temple University, Philadelphia, PA 19122, USA.

Cells
|October 14, 2022
PubMed
Summary

Imaging transient RNA in live cells is challenging. Recent advances offer new methods for labeling and visualizing endogenous RNA at the single-molecule level using super-resolution microscopy.

Keywords:
CRISPR-Cas13CRISPR-Cas9FISHMS2-MCPMTRIPsSMLMmRNAmolecular beaconsseqFISHsingle-molecule super-resolution microscopysmFISH

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Microscopy

Background:

  • RNA's transient nature presents significant challenges for biological imaging.
  • Difficulty in imaging endogenous RNA within transfected cells complicates research.
  • Traditional super-resolution microscopy faces hurdles in RNA visualization.

Purpose of the Study:

  • To review current techniques for labeling and imaging RNA.
  • To highlight advancements in live-cell RNA imaging.
  • To focus on labeling methods and 3D super-resolution imaging.

Main Methods:

  • Review of various RNA labeling strategies.
  • Discussion of techniques for imaging endogenous RNA in live cells.
  • Exploration of 3D super-resolution imaging approaches.

Main Results:

  • Recent advances enable cellular and single-molecule level imaging of endogenous RNA.
  • New tools facilitate overcoming previous limitations in RNA visualization.
  • Effective labeling and advanced microscopy are key to successful imaging.

Conclusions:

  • Live-cell imaging of endogenous RNA is now feasible with advanced techniques.
  • Progress in labeling and super-resolution microscopy enhances RNA visualization.
  • This review provides insights into state-of-the-art RNA imaging methodologies.