Photochemical Consideration in the Interactions between Blood Proteins and Layered Inorganic Materials
Tetsuo Yamaguchi1, Hyoung-Mi Kim2, Jae-Min Oh1
1Department of Energy and Materials Engineering, Dongguk University, Seoul 04620, Korea.
International Journal of Molecular Sciences
|October 14, 2022
Summary
Layered double hydroxide (LDH) nanomaterials show weak interactions with human blood plasma proteins. Surface charge significantly influences these interactions, suggesting good biocompatibility for biomedical applications.
Area of Science:
- Nanomaterials Science
- Biomaterials Engineering
- Surface Chemistry
Background:
- Layered double hydroxides (LDHs) are promising nanomaterials for biomedical applications.
- Understanding nanomaterial-protein interactions is crucial for predicting in vivo behavior and biocompatibility.
- Particle size and surface charge are key factors influencing nanomaterial-biomolecule interactions.
Purpose of the Study:
- To investigate the interactions between various layered double hydroxide (LDH) nanomaterials and human blood plasma proteins.
- To evaluate the influence of LDH particle size and surface charge on these interactions.
- To assess the potential biocompatibility of LDH nanomaterials in a biological context.
Main Methods:
- Preparation of LDHs with controlled particle sizes (150, 350, 2000 nm) via hydrothermal treatment and urea hydrolysis.
- Surface modification of LDHs using organic coatings (citrate, malite, serite) to achieve specific surface charges (ζ-potential: -15, 6, 36 mV).
- Analysis of nanomaterial-protein interactions using adsorption isotherms and fluorescence quenching assays with human blood plasma and specific proteins (albumin, globulin, fibrinogen).
Main Results:
- Weak interactions were observed between all tested LDH nanomaterials and human blood plasma.
- Adsorption isotherms showed no significant differences based on particle size or surface charge.
- Fluorescence quenching increased with higher surface charges, indicating a major role for electrostatic interactions in protein adsorption. A single layer of protein adsorbed onto LDHs, with additional proteins weakly associating.
Conclusions:
- LDH nanomaterials exhibit fair compatibility with blood components due to protein corona formation.
- Electrostatic interactions play a significant role in modulating the interaction between LDHs and plasma proteins.
- Surface charge is a critical parameter for controlling LDH-protein interactions and assessing biocompatibility.
Keywords:
biocompatibilityfibrinogenfluorescence quenchinghuman serum albuminlayered double hydroxideγ-globulinMore Related Videos
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