Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

870
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
870
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.7K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.7K
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

5.6K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
5.6K
Mass Spectrometry: Carboxylic Acid, Ester, and Amide Fragmentation01:01

Mass Spectrometry: Carboxylic Acid, Ester, and Amide Fragmentation

1.5K
The fragmentation patterns observed for compounds such as carboxylic acids, esters, and amides in the mass spectra include ⍺-cleavage and McLafferty rearrangement. Fragmentation by ⍺-cleavage preferentially occurs at the carbon-carbon bond at the ⍺-position next to the carboxylic group to generate a neutral radical and a cation. Long chain compounds with hydrogen at their γ-carbon undergo McLafferty rearrangement to give a radical cation and a neutral alkene.
For example,...
1.5K
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

5.1K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
5.1K
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

1.5K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
1.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Functionalization of Saturated and Unsaturated Hydrocarbons with Electrophilic Anions [B<sub>12</sub>X<sub>11</sub>]<sup>-</sup> (X = Br, I) in the Gas Phase and at Interfaces.

The journal of physical chemistry. A·2026
Same author

Inhibition of N6-Methyladenosine Accumulation by Targeting METTL3 Mitigates Tau Pathology and Cognitive Decline in Alzheimer's Disease.

Research square·2026
Same author

Effects of Amino-Substitution on the Gas-Phase Reactivity of Quinoline-Based Singlet Oxenium Cations.

The Journal of organic chemistry·2025
Same author

Gas-Phase Reactivity of Quinoline-Based Singlet Oxenium Cations.

The Journal of organic chemistry·2024
Same author

Spatial Lipidomics Maps Brain Alterations Associated with Mild Traumatic Brain Injury.

bioRxiv : the preprint server for biology·2024
Same author

Ultrahigh Resolution Lipid Mass Spectrometry Imaging of High-Grade Serous Ovarian Cancer Mouse Models.

bioRxiv : the preprint server for biology·2023

Related Experiment Video

Updated: Aug 25, 2025

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

19.8K

Recent Advances in Mass Spectrometry-Based Structural Elucidation Techniques.

Xin Ma1

  • 1School of Chemistry and Biochemistry, Georgia Institute of Technology, 901 Atlantic Dr NW, Atlanta, GA 30332, USA.

Molecules (Basel, Switzerland)
|October 14, 2022
PubMed
Summary

Mass spectrometry (MS) is key for analyzing molecular structures. Recent technical advances enhance its use in complex mixture analysis for biomolecules and small compounds.

Keywords:
gas-phase ion chemistrymolecular characterizationomicsstructural elucidationtandem mass spectrometry

More Related Videos

Analyzing Large Protein Complexes by Structural Mass Spectrometry
15:35

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 19, 2010

24.3K
Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
07:33

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Published on: October 15, 2018

14.4K

Related Experiment Videos

Last Updated: Aug 25, 2025

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

19.8K
Analyzing Large Protein Complexes by Structural Mass Spectrometry
15:35

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 19, 2010

24.3K
Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
07:33

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Published on: October 15, 2018

14.4K

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Structural Biology

Background:

  • Mass spectrometry (MS) is a primary technique for gas-phase molecular structure analysis.
  • High-resolution MS provides accurate molecular weights for confident molecular formula assignment.
  • Tandem MS enables detailed structural characterization through fragmentation analysis.

Purpose of the Study:

  • To review technical advances in MS-based structural elucidation over the past five years.
  • To provide an overview of MS applications in complex mixture analysis.
  • To make MS techniques accessible to a broader audience.

Main Methods:

  • High-resolution mass spectrometry for accurate mass measurement.
  • Tandem mass spectrometry for fragmentation analysis.
  • Coupling MS with separation techniques (chromatography, ion mobility) and NMR.

Main Results:

  • MS is effective for structural elucidation of small molecules and biopolymers.
  • High sensitivity allows analysis of low-level analytes in complex matrices.
  • MS is versatile and can be coupled with various analytical instruments.

Conclusions:

  • Recent advances have significantly improved MS-based structural elucidation.
  • MS is crucial for multiomics studies, revealing biomolecular structures, functions, and interactions.
  • The review highlights the expanding applications of MS in analyzing complex mixtures.