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Compact Quantum Dots for Single-molecule Imaging

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Published on: October 9, 2012

Near Infrared Emitting Semiconductor Polymer Dots for Bioimaging and Sensing.

Connor Riahin¹, Kushani Mendis¹, Brandon Busick¹

¹Department of Chemistry and Biochemistry, University of Maryland Baltimore County, Baltimore, MD 21250, USA.

Sensors (Basel, Switzerland)

|October 14, 2022

Summary

Semiconducting polymer dots (Pdots) offer advanced fluorescence bioimaging and sensing. Dye-doped Pdots enable deep red/near-infrared imaging and analyte detection, with new near-infrared emitting Pdots also developed.

Keywords:

near infrared emission plant cell imaging polymer dots sensing

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Synthesis of Cd-free InP/ZnS Quantum Dots Suitable for Biomedical Applications

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Published on: February 6, 2016

Area of Science:

Nanotechnology
Materials Science
Biomedical Engineering

Background:

Semiconducting polymer dots (Pdots) are emerging as versatile nanoparticles for bioimaging and sensing.
Their photophysical properties, including high brightness and photostability, make them attractive fluorophores.
Modifying Pdots with dopant molecules enhances their optical properties for specific applications.

Purpose of the Study:

To advance fluorescence bioimaging and sensing using nanoscale luminescent Pdots.
To explore the use of dye dopants for deep red and near-infrared (NIR) fluorescence applications.
To develop Pdots sensitive to specific analytes and for oxygen sensing.

Main Methods:

Synthesis of dye-doped semiconducting polymer dots (Pdots).
Conjugation of secondary antibodies to Pdots for targeted delivery.
Characterization using Cryo-Transmission Electron Microscopy (Cryo-TEM).
Demonstration of targeted cellular imaging and sensing in aqueous media.

Main Results:

Dye-doped Pdots exhibit tunable optical properties for deep red and NIR fluorescence.
Antibody-conjugated Pdots selectively target FLAG-tagged FLS2 membrane receptors in plant cells.
Novel Pdots emitting around 1000 nm were successfully synthesized.
Oxygen-sensitive Pdots were developed and demonstrated in aqueous solutions.

Conclusions:

Dye-doped Pdots are highly adaptable for advanced fluorescence bioimaging and sensing.
Antibody conjugation enables targeted cellular imaging applications.
Development of NIR-emitting and oxygen-sensing Pdots expands their utility in biological and environmental monitoring.