Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Depth Perception and Spatial Vision

Depth Perception and Spatial Vision

Depth perception is the ability to perceive objects three-dimensionally. It relies on two types of cues: binocular and monocular. Binocular cues depend on the combination of images from both eyes and how the eyes work together. Since the eyes are in slightly different positions, each eye captures a slightly different image. This disparity between images, known as binocular disparity, helps the brain interpret depth. When the brain compares these images, it determines the distance to an object.

Linear Approximation in Frequency Domain

Linear Approximation in Frequency Domain

Linear systems are characterized by two main properties: superposition and homogeneity. Superposition allows the response to multiple inputs to be the sum of the responses to each individual input. Homogeneity ensures that scaling an input by a scalar results in the response being scaled by the same scalar.
In contrast, nonlinear systems do not inherently possess these properties. However, for small deviations around an operating point, a nonlinear system can often be approximated as linear....

Linear Approximation in Time Domain

Linear Approximation in Time Domain

Nonlinear systems often require sophisticated approaches for accurate modeling and analysis, with state-space representation being particularly effective. This method is especially useful for systems where variables and parameters vary with time or operating conditions, such as in a simple pendulum or a translational mechanical system with nonlinear springs.
For a simple pendulum with a mass evenly distributed along its length and the center of mass located at half the pendulum's length,...

Deconvolution

Deconvolution

Deconvolution, also known as inverse filtering, is the process of extracting the impulse response from known input and output signals. This technique is vital in scenarios where the system's characteristics are unknown, and they must be inferred from the observable signals.
Deconvolution involves several mathematical techniques to derive the impulse response. One common approach is polynomial division. In this method, the input and output sequences are treated as coefficients of...

Distance Measurements by Taping

Distance Measurements by Taping

Tapes are essential in surveying for accurate, durable, and short-distance measurements. Made from lightweight, nylon-coated steel, they offer flexibility and strength for rugged outdoor use. The nylon coating protects against rust and wear, extending the tape's life. Standard lengths, around 30 meters, are marked in meters and millimeters for precision.Surveyors select tapes based on site conditions and accuracy needs. Lightweight, nylon-coated tapes are commonly used for ease of handling and...

Downsampling

Downsampling

When considering a sampled sequence with zero values between sampling instants, one can replace it by taking every N-th value of the sequence. At these integer multiples of N, the original and sampled sequences coincide. This process, known as decimation, involves extracting every N-th sample from a sequence, thereby creating a more efficient sequence.
The Fourier transform of the decimated sequence reveals a combination of scaled and shifted versions of the original spectrum. This...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

UniOCTSeg++: Refined Hierarchical Prompt Strategy and Bi-directional Progressive Consistency Learning for Universal Retinal Layer Segmentation in OCT.

IEEE transactions on medical imaging·2026

Same author

High-performance surface plasmon resonance sensor utilizing AuNPs/Au nanocone array for ultrasensitive dopamine detection in complex biological matrices.

Mikrochimica acta·2026

Same author

Flat-top dual-pump supercontinuum laser in the NIR-IIc region and its applications in spectroscopic analysis of diverse polymers.

Optics express·2026

Same author

All-Optical Diffractive Operators for Rapid, Computer-Free Morphological Transformations.

Nanophotonics (Berlin, Germany)·2026

Same author

Preliminary application and efficacy evaluation of the modified Lich-Gregoir technique in the management of primary obstructive megaureter.

Journal of pediatric surgery·2026

Same author

SynLiTS: Phase Prompting-Driven Diffusion Synthesis and Context-Aware Fusion for Unaligned Liver Tumor Segmentation.

IEEE transactions on medical imaging·2026

Search research articles

Related Experiment Video

Updated: Aug 25, 2025

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

Published on: December 3, 2013

Quantized spiral-phase-modulation based deep learning for real-time defocusing distance prediction.

Zezheng Zhang, Ryan K Y Chan, Kenneth K Y Wong

|October 14, 2022

Summary

This summary is machine-generated.

Whole slide imaging (WSI) requires precise sample positioning. This study introduces a novel autofocusing method using quantized spiral phase modulation and a lightweight neural network, significantly improving accuracy and speed for pathology diagnostics.

More Related Videos

Lensless Fluorescent Microscopy on a Chip

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

Related Experiment Videos

Last Updated: Aug 25, 2025

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

Published on: December 3, 2013

Lensless Fluorescent Microscopy on a Chip

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

Area of Science:

Digital pathology
Computational imaging
Optical microscopy

Background:

Whole slide imaging (WSI) is crucial for remote and collaborative pathological diagnosis.
Achieving optimal sample positioning and artifact-free imaging in WSI remains a challenge.
Traditional autofocusing methods for WSI are often not universal or are time-consuming.

Purpose of the Study:

To develop a more efficient and accurate autofocusing method for whole slide imaging.
To address the challenge of sample positioning and defocusing artifacts in WSI.
To enable real-time autofocusing on edge devices with limited resources.

Main Methods:

Application of quantized spiral phase modulation in the Fourier domain of captured images.
Utilizing a lightweight neural network for predicting defocusing distance.
Evaluation on an open dataset to assess prediction error and speed.

Main Results:

Significant reduction in average prediction error compared to previous methods.
Achieved prediction error lower than any reported on the open dataset.
Demonstrated high prediction speed suitable for real-time applications.

Conclusions:

The proposed method offers a highly accurate and fast autofocusing solution for WSI.
Quantized spiral phase modulation combined with a lightweight neural network overcomes limitations of traditional methods.
The approach is suitable for deployment on edge devices, enhancing the efficiency of digital pathology workflows.