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ISG15 is associated with cervical cancer development
Pingping Tao1, Liyan Sun1, Yanmei Sun1
1Department of Obstetrics and Gynecology, Pudong New Area People's Hospital Affiliated to Shanghai Health University, Shanghai 201299, P.R. China.
Oncology Letters
|October 14, 2022
Summary
Interferon-stimulated gene 15 (ISG15) is upregulated in cervical cancer (CC) and promotes tumor growth. Inhibiting ISG15 may offer a new therapeutic strategy for CC.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Cervical cancer (CC) pathogenesis involves complex molecular mechanisms.
- Understanding transcriptomic alterations is crucial for identifying CC drivers.
Purpose of the Study:
- To investigate transcriptomic differences in cervical cancer.
- To explore the role of ISG15 ubiquitin-like modifier (ISG15) in CC progression.
- To simulate tumor progression and identify key molecular events.
Main Methods:
- RNA sequencing for transcriptomic analysis of normal, paracarcinoma, and tumor tissues.
- Pseudo-time analysis to simulate tumor progression.
- RT-qPCR and immunohistochemistry to quantify ISG15 expression.
- Cell proliferation, wound healing, and Transwell assays to assess ISG15 function.
Main Results:
- ISG15 expression was significantly upregulated in cervical tumor tissues compared to normal and paracarcinoma tissues.
- A progressive increase in ISG15 expression was observed from normal to paracarcinoma to tumor tissues.
- ISG15 inhibition using small interfering RNA reduced proliferation and invasion in HeLa cervical cancer cells.
Conclusions:
- ISG15 is upregulated in cervical cancer and positively correlates with tumor development.
- ISG15 may function as an oncogene in cervical cancer tumorigenesis.
- Targeting ISG15 could be a potential therapeutic strategy for cervical cancer.
