Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

6.5K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
6.5K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.9K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Programmable artificial RNA condensates in mammalian cells.

Nature nanotechnology·2026
Same author

Molecular recruitment and release using DNA host condensates.

Nanoscale horizons·2026
Same author

Programmable artificial RNA condensates in mammalian cells.

bioRxiv : the preprint server for biology·2026
Same author

Internal Phase Separation in Synthetic DNA Condensates.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Author Correction: Modular RNA motifs for orthogonal phase separated compartments.

Nature communications·2025
Same author

Switchable RNA motifs for dynamic transcriptional control of RNA condensates.

Nucleic acids research·2025

Related Experiment Video

Updated: Aug 25, 2025

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
08:15

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Published on: June 26, 2020

4.3K

Fueling DNA Self-Assembly via Gel-Released Regulators.

Jenny Le1, Dino Osmanovic1, Melissa Ann Klocke1

  • 1Department of Mechanical and Aerospace Engineering, University of California at Los Angeles, Los Angeles90095, United States.

ACS Nano
|October 14, 2022
PubMed
Summary

Researchers developed novel hydrogel mini-gels to precisely control DNA nanotube self-assembly. These compartments allow for timed release of DNA and localized reactions, enabling sophisticated biomolecular material construction.

Keywords:
DNA nanotechnologyDNA nanotubesDNA tilesUV irradiationhydrogelsphotocleavagepolyacrylamide

More Related Videos

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

14.5K
Preparation of DNA-crosslinked Polyacrylamide Hydrogels
09:06

Preparation of DNA-crosslinked Polyacrylamide Hydrogels

Published on: August 27, 2014

14.8K

Related Experiment Videos

Last Updated: Aug 25, 2025

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
08:15

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Published on: June 26, 2020

4.3K
Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

14.5K
Preparation of DNA-crosslinked Polyacrylamide Hydrogels
09:06

Preparation of DNA-crosslinked Polyacrylamide Hydrogels

Published on: August 27, 2014

14.8K

Area of Science:

  • Biomolecular Engineering
  • Materials Science
  • Synthetic Biology

Background:

  • Developing responsive molecular materials necessitates methods for spatially separating and dynamically coupling distinct chemical processes.
  • Controlling self-assembly in multicomponent systems is crucial for advanced material design.

Purpose of the Study:

  • To demonstrate the use of hydrogel mini-gels as controllable compartments for DNA release and localized reactions.
  • To achieve precise control over the kinetics of DNA nanotube assembly and disassembly.

Main Methods:

  • Utilizing microliter-sized polyacrylamide hydrogels (mini-gels) loaded with DNA molecules.
  • Characterizing UV-mediated DNA release from mini-gels to control diffusion rates and minimize leakage.
  • Employing mini-gels for one-pot storage and release of DNA regulators to mediate nanotube assembly/disassembly.
  • Localizing enzymatic reactions within mini-gels for in-situ RNA regulator transcription.

Main Results:

  • Demonstrated controlled release of DNA from mini-gels with tunable kinetics based on gel porosity.
  • Achieved precise control over DNA nanotube assembly and disassembly kinetics, including introducing delays.
  • Successfully localized enzymatic transcription of RNA regulators within mini-gels, triggering self-assembly upon diffusion.
  • Validated experimental findings with a mathematical model describing DNA diffusion and its effect on self-assembly.

Conclusions:

  • Hydrogel mini-gels offer a versatile platform for compartmentalizing and controlling molecular processes.
  • This approach enables dynamic self-assembly of DNA nanotubes with tunable kinetics and spatiotemporal control.
  • Mini-gels hold potential for constructing complex biomolecular materials for biomedical applications and artificial life.