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Affinity Chromatography

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...

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Exploring multifunctional components from Andrographis paniculata by affinity ultrafiltration with three molecular

Huixia Feng¹, Guilin Chen¹, Mingquan Guo¹

¹Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China; University of Chinese Academy of Sciences, Beijing 100049, China; Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 430074, China; Innovation Academy for Drug Discovery and Development, Chinese Academy of Sciences, Shanghai 201203, China.

|October 14, 2022

Summary

Andrographis paniculata, a medicinal plant, contains compounds that may target inflammation and viral replication. Researchers identified potential bioactive molecules using a novel screening method, aiding future drug discovery.

Keywords:

Andrographis paniculata Anti-inflammatory activity Anti-viral activity UF-LC/MS screening

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Area of Science:

Phytochemistry
Pharmacology
Natural Product Chemistry

Background:

Andrographis paniculata (AP) is a plant used in traditional medicine with known immune-regulatory, anti-inflammatory, and anti-viral properties.
The specific bioactive components and their mechanisms of action within AP remain largely uncharacterized.
Understanding these mechanisms is crucial for developing AP-derived therapeutics.

Purpose of the Study:

To screen for potential bioactive compounds in Andrographis paniculata.
To identify specific compounds that target key inflammatory and viral pathway proteins, such as COX-2, IL-6, and ACE2.
To elucidate the mechanisms underlying AP's therapeutic effects.

Main Methods:

Utilized a combination of bio-affinity ultrafiltration with liquid chromatography-mass spectrometry (UF-LC/MS) for high-throughput screening.
Employed other bioassays to assess the inhibitory effects of AP crude extract on COX-2 and ACE2.
Performed molecular docking analysis to evaluate the binding affinities of identified compounds to target proteins.

Main Results:

The crude extract of AP demonstrated inhibitory activity against COX-2 and ACE2.
Eleven potential ligands targeting COX-2, IL-6, and ACE2 were identified from AP.
Andrographolide and 14-deoxy-11,12-didehydroandrographolide showed strong binding affinities to COX-2, IL-6, and ACE2, confirmed by UF-LC/MS and molecular docking.

Conclusions:

This study successfully applied the UF-LC/MS approach to rapidly screen multi-target ligands from Andrographis paniculata.
Identified andrographolide and its derivative as promising compounds with potential therapeutic applications.
The findings provide a foundation for further research into AP's bioactive compounds and their mechanisms, potentially accelerating the discovery of new drugs from natural sources.