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pMTL60000: A modular plasmid vector series for Parageobacillus thermoglucosidasius strain engineering
Abubakar Madika1, Jennifer Spencer2, Matthew S H Lau2
1BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Biodiscovery Institute, The University of Nottingham, Nottingham NG7 2RD, United Kingdom; Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria, Nigeria.
Abstract:
Parageobacillus thermoglucosidasius is a promising chassis for producing chemicals and fuels. Here we designed, built and tested the pMTL60000 modular plasmids containing standardised Gram-positive and Gram-negative replicons, selectable markers and application-specific modules. The pMTL60000 modular plasmids were characterised with regard to transformation efficiency, segregational stability, copy number and compatibility.
