Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Environmental DNA: From Promise to Practice.

Ecology and evolution·2026
Same author

Standardizing Primary Care Management of Comorbidities in Youth with Overweight or Obesity Using Technological and Visual Aids to Facilitate Evaluation and Support for Healthy Lifestyle Change.

Childhood obesity (Print)·2026
Same author

Accelerated ibuprofen removal in soils via bioaugmentation: Insights into the involved microbiomes.

Journal of hazardous materials·2026
Same author

Candida parapsilosis dominates the surface-colonizing yeast community in the hospital environment as revealed by a combined culture and environmental DNA approach.

The Journal of hospital infection·2026
Same author

Lifestyle Medicine Bootcamp Improves Medical Student Understanding, Confidence, and Intent in Clerkships.

American journal of lifestyle medicine·2026
Same author

Single-cell analysis of a salmonid immune system (river brown trout Salmo trutta fario) reveals evolutionary divergence and hatchery-induced transcriptional reprogramming.

BMC biology·2026

Related Experiment Video

Updated: Aug 25, 2025

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
12:14

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

Published on: March 9, 2016

9.9K

Detecting aquatic pathogens with field-compatible dried qPCR assays.

Jessica Rieder1, Pedro M Martin-Sanchez2, Omneya A Osman3

  • 1Institute for Fish and Wildlife Health, University of Bern, Länggassstrasse 122, 3012 Bern, Switzerland.

Journal of Microbiological Methods
|October 14, 2022
PubMed
Summary

New qPCR assays for aquatic pathogens Gyrodactylus salaris and Aphanomyces astaci have extended shelf-lives. Lyophilization and air-drying methods support rapid monitoring and conservation efforts for these critical fish pathogens.

Keywords:
Air-dried assayAquatic pathogensEnvironmental diagnosticsLyophilizationeDNAqPCR

More Related Videos

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
08:54

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

Published on: November 5, 2020

14.1K
Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification
07:25

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

Published on: June 25, 2020

6.6K

Related Experiment Videos

Last Updated: Aug 25, 2025

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
12:14

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

Published on: March 9, 2016

9.9K
Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
08:54

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

Published on: November 5, 2020

14.1K
Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification
07:25

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

Published on: June 25, 2020

6.6K

Area of Science:

  • Aquatic microbiology
  • Molecular diagnostics
  • Conservation biology

Background:

  • Emerging aquatic pathogens pose significant threats to biodiversity and ecosystem health.
  • Effective monitoring programs are crucial for timely conservation and management interventions.
  • Existing diagnostic tools often lack the stability required for field deployment.

Purpose of the Study:

  • To develop and validate shelf-stable quantitative polymerase chain reaction (qPCR) assays.
  • To target key aquatic pathogens: Gyrodactylus salaris (a salmonid parasite) and Aphanomyces astaci (an oomycete causing crayfish plague).
  • To assess the impact of lyophilization and air-drying on assay stability and performance.

Main Methods:

  • Development of field-ready qPCR assays.
  • Validation of assay accuracy and sensitivity.
  • Testing of extended shelf-life using lyophilization and air-drying techniques.
  • Targeting of Gyrodactylus salaris and Aphanomyces astaci DNA.

Main Results:

  • Successfully developed and validated qPCR assays for Gyrodactylus salaris and Aphanomyces astaci.
  • Demonstrated extended shelf-life for both assays when preserved using lyophilization and air-drying.
  • Confirmed the reliability of these methods for field applications.

Conclusions:

  • Lyophilization and air-drying are effective methods for creating stable, field-ready qPCR assays.
  • These enhanced assays will improve monitoring programs for critical aquatic pathogens.
  • The developed assays will facilitate rapid decision-making for aquatic conservation and management.