Detecting aquatic pathogens with field-compatible dried qPCR assays
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Updated: Aug 25, 2025

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A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
Published on: March 9, 2016
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Jessica Rieder1, Pedro M Martin-Sanchez2, Omneya A Osman3
1Institute for Fish and Wildlife Health, University of Bern, Länggassstrasse 122, 3012 Bern, Switzerland.
Journal of Microbiological Methods
|October 14, 2022
Abstract:
Field-ready qPCR assays with extended shelf-life support monitoring programs for emerging aquatic pathogens and enable quick conservation and management decisions. Here, we developed, validated, and tested the shelf-life of qPCR assays targeting Gyrodactylus salaris and Aphanomyces astaci with lyophilization and air-drying.

