Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ionic strength and myofibrillar protein solubilization.

F Y Wu, S B Smith

    Journal of Animal Science
    |August 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Targeting Psoroptes ovis MIF to improve skin barrier function: A novel prophylactic strategy for psoroptic mange.

    Veterinary parasitology·2026
    Same author

    Recombinant PsoSP4 confers superior 80% protection against psoroptic mange in rabbits in association with a Th1-biased immune response.

    Veterinary parasitology·2026
    Same author

    Calibration of a gated neutron time-of-flight spectrometer with low-afterglow.

    The Review of scientific instruments·2025
    Same author

    DNA calorimetric force spectroscopy at single base pair resolution.

    Nature communications·2025
    Same author

    Application two-dimensional gel electrophoresis coupled with LC-MS/MS to identify candidate serodiagnostic antigens for early detection Psoroptes ovis var. cuniculi infection.

    Veterinary parasitology·2024
    Same author

    [Progress in platelets and tuberculosis].

    Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2024
    Same journal

    Water intake and consumption behaviour of colony and privately-owned healthy domestic cats fed 100% dry, 50% dry and 50% wet, and 100% wet diets: A comparison of research and home environments.

    Journal of animal science·2026
    Same journal

    Gestational Vitamin and Mineral Supplementation in F0 Beef Heifers: Impacts on F1 Heifer Nutrient Digestibility, Metabolic Balance, and F1 Dam and F2 Fetus Development.

    Journal of animal science·2026
    Same journal

    A self-reinforcing transcriptional loop: ELF5 directly activates the STAT5B promoter to orchestrate milk protein synthesis in water buffalo.

    Journal of animal science·2026
    Same journal

    Provision of porcine milk oligosaccharides to support the weaning transition in nursery pigs fed diets including bovine milk co-products.

    Journal of animal science·2026
    Same journal

    The effects of feed intake and fiber type on apparent ileal and total tract digestibility and hindgut disappearance of energy and nutrients in growing pigs.

    Journal of animal science·2026
    Same journal

    Effects of dietary octapeptin supplementation on growth performance, intestinal morphology, immune function, and serum metabolism of weaned piglets.

    Journal of animal science·2026
    See all related articles

    High ionic strength and extended incubation solubilize bovine myofibrils, releasing key proteins like actin and myosin. pH 5.5 further impacts protein release and myofibril structure.

    Area of Science:

    • Meat Science
    • Protein Biochemistry
    • Muscle Biology

    Background:

    • Myofibrils are the primary contractile units in muscle tissue.
    • Understanding myofibrillar protein behavior is crucial for meat quality and processing.

    Purpose of the Study:

    • To investigate the effects of ionic strength, incubation time, and pH on bovine myofibril structure and protein solubility.
    • To identify specific myofibrillar proteins affected by these in vitro conditions.

    Main Methods:

    • Incubation of bovine longissimus muscle myofibrils in varying ionic strength buffers (0.10–0.35 M KCl or NaCl).
    • Analysis of protein solubilization and suspension turbidity over time (1–72 h).
    • Gel electrophoresis (SDS-PAGE) to identify released proteins; electron microscopy for structural changes.

    Related Experiment Videos

    Main Results:

    • Increased ionic strength and incubation time led to decreased turbidity and increased myofibrillar solubilization.
    • KCl and NaCl effectively dissociated M-protein, actin, troponin-T, tropomyosin, and myosin light chain-3.
    • pH 5.5 significantly altered the release of specific proteins compared to pH 7.0.
    • Thick filament ultrastructure degraded over time, while Z-lines were more resistant unless treated with trypsin.

    Conclusions:

    • Ionic strength and incubation time are key factors influencing myofibrillar protein solubility and structure.
    • Specific protein dissociation patterns vary with salt type and pH.
    • These findings provide insights into in vitro muscle protein behavior relevant to meat science.