Enhanced nitrate, fluoride, and phenol removal using polyurethane sponges loaded with rice husk biochar in...

Polyurethane sponges loaded with rice husk biochar were prepared to immobilize Aquabacterium sp. CZ3 for intensified removal of nitrate, fluoride (F^-), and phenol, with the maximum efficiency of 100 %, 91 %, and 99 %, respectively. The biochar load and increased carbon-to-nitrogen (C:N) ratio (below 3.0) stimulated the secretion of soluble microbial product, improved the electron transport system activity, and promoted denitrification, phenol co-metabolism, and F^- and calcium crystallization. The characterization results suggested that F^- was removed as fluoride-containing calcium precipitates. According to the microbial community analyses, Aquabacterium was the dominant bacterium. PICRUSt analyses showed that biochar and adequate carbon sources (C:N ratio 3.0) significantly increased the functional abundances of amino acid metabolism, carbohydrate metabolism, energy metabolism, and cell motility. The introduction of biochar reduces the demand for C:N ratio in the system, and expands the application potential of biomineralization technique in the remediation of multiple pollutants contaminated water.