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Updated: Aug 25, 2025
High-throughput Saccharification Assay for Lignocellulosic Materials
Published on: July 3, 2011
Deconstructing sugarcane bagasse lignocellulose by acid-based deep eutectic solvents to enhance enzymatic
María Guadalupe Morán-Aguilar1, Montserrat Calderón-Santoyo2, Ricardo Pinheiro de Souza Oliveira3
1Industrial Biotechnology and Environmental Engineering Group "BiotecnIA", Chemical Engineering Department, University of Vigo (Campus Ourense), 32004 Ourense, Spain; Tecnológico Nacional de México/I. T. de Tepic, Integral Food Research Laboratory, C.P. 63175 Tepic, Nayarit, Mexico.
Abstract:
Biorefinery with deep eutectic solvent (DES) is an emerging processing technology to overcome the shortcomings of conventional biomass pretreatments. This work evaluates the biorefinery of sugarcane bagasse (SCB) with DES formulated with choline chloride as hydrogen bond acceptor and three hydrogen bond donors: lactic acid, citric acid, and acetic acid. Acetic acid showed unique ionic properties responsible for the selective removal of lignin and the deconstruction of cellulose to improve the digestibility of up to 97.61 % of glucan and 63.95 % of xylan during enzymatic hydrolysis. In addition, the structural characteristics of the polysaccharide-rich material (PRM) were analyzed by X-rays, ATR-FTIR, SEM, and enzymatic hydrolysis, and compared with the original material sample, for a comprehensive understanding of biomass deconstruction using different hydrogen bond donors (HBD) as DES pretreatment.
