Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fiber type specific glycogen utilization in rat diaphragm during treadmill exercise.

H J Green, M E Ball-Burnett, M A Morrissey

    Journal of Applied Physiology (Bethesda, Md. : 1985)
    |July 1, 1987
    PubMed
    Summary

    Endogenous glycogen is a crucial fuel source for diaphragm muscle fibers during intense exercise. Glycogen depletion occurs rapidly, with full recovery achieved within four hours in most muscles.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Lower body negative pressure identifies altered central vein characteristics without accompanying changes to baroreflexes in astronauts within hours of landing.

    Scientific reports·2024
    Same author

    Intermittent compression of the calf muscle as a countermeasure to protect blood pressure and brain blood flow in upright posture in older adults.

    European journal of applied physiology·2021
    Same author

    Efficacy of a web-based cognitive rehabilitation intervention for adult cancer survivors: A pilot study.

    European journal of cancer care·2018
    Same author

    Enhanced muscle blood flow with intermittent pneumatic compression of the lower leg during plantar flexion exercise and recovery.

    Journal of applied physiology (Bethesda, Md. : 1985)·2017
    Same author

    Prediction of oxygen uptake dynamics by machine learning analysis of wearable sensors during activities of daily living.

    Scientific reports·2017
    Same author

    Linear and non-linear contributions to oxygen transport and utilization during moderate random exercise in humans.

    Experimental physiology·2017

    Area of Science:

    • Exercise Physiology
    • Skeletal Muscle Metabolism
    • Respiratory Muscle Function

    Background:

    • Endogenous glycogen stores are vital for sustained muscle activity.
    • The diaphragm's specific fiber types and their metabolic responses to exercise require further investigation.
    • Understanding substrate utilization during prolonged exercise is critical for performance and fatigue.

    Purpose of the Study:

    • To investigate the role of endogenous glycogen in different diaphragm muscle fiber types (Type I, IIa, IIb) during exhaustive exercise.
    • To compare glycogen utilization and repletion patterns in the diaphragm with other skeletal muscles (soleus, plantaris, vastus lateralis).
    • To determine the significance of glycogen as an energy substrate for the diaphragm during the work of breathing.

    Main Methods:

    Related Experiment Videos

    • Adult male Wistar rats underwent treadmill running protocols of 30 minutes or until exhaustion.
    • Microphotometric analysis using the periodic acid-Schiff technique quantified glycogen content in diaphragm muscle fibers.
    • Biochemical assays assessed glycogen concentrations in diaphragm and limb muscles.
    • Glycogen repletion was monitored during a 4-hour recovery period.

    Main Results:

    • Significant glycogen depletion (73-80%) was observed in all diaphragm fiber types within 30 minutes of exercise.
    • No further glycogen reduction occurred in the diaphragm after reaching exhaustion (additional 94 minutes of exercise).
    • Substantial glycogen reductions were noted in soleus, plantaris, and red vastus lateralis, with less pronounced depletion in white vastus lateralis.
    • Glycogen repletion was complete within 4 hours in all muscles except the white vastus lateralis.

    Conclusions:

    • Endogenous glycogen serves as a significant substrate for all sampled muscles, irrespective of fiber composition.
    • The diaphragm's costal region recruits all fiber types during heavy exercise, relying on glycogen as a primary substrate early in the activity.
    • These findings highlight the critical role of glycogen in supporting respiratory muscle function during strenuous physical exertion.