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Interlaboratory study of blood selenium determinations.

T S Koh

    Journal - Association of Official Analytical Chemists
    |July 1, 1987
    PubMed
    Summary
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    This study highlights challenges in accurately measuring selenium (Se) in bovine blood, particularly at low concentrations relevant for diagnosing livestock deficiency. High interlaboratory variability and ineffective quality control at low Se levels are key concerns.

    Area of Science:

    • Veterinary Medicine
    • Analytical Chemistry
    • Biochemistry

    Background:

    • Accurate determination of selenium (Se) in bovine blood is crucial for diagnosing Se deficiency in livestock.
    • Existing analytical methods show significant variability, especially at low concentrations.
    • Quality control (QC) schemes may not adequately address interlaboratory variability at clinically relevant Se levels.

    Purpose of the Study:

    • To assess the performance of different analytical methods for selenium determination in bovine blood across multiple laboratories.
    • To evaluate the impact of laboratory participation and quality control on the accuracy and precision of Se measurements.
    • To identify challenges in diagnosing marginal selenium deficiency in livestock due to analytical variability.

    Main Methods:

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    • A survey involving 51 laboratories from 14 countries.
    • Analysis of 8 bovine blood samples with Se concentrations ranging from 0.2 to 14 mumol/L.
    • Utilized methods included fluorometry, hydride-generation atomic absorption spectrophotometry (AAS), graphite-furnace AAS, gas chromatography, neutron activation analysis, and X-ray fluorometry.

    Main Results:

    • Fluorometry and hydride-generation AAS showed comparable mean Se results (P>0.05).
    • Intralaboratory coefficients of variation (CVs) ranged from 4-14%.
    • Interlaboratory CVs increased significantly with decreasing Se concentration, reaching 55% below 0.4 mumol/L.
    • QC schemes showed limited effectiveness in reducing interlaboratory CVs at low Se concentrations.

    Conclusions:

    • High interlaboratory variability and ineffective QC at low Se concentrations pose a risk for accurate diagnosis of marginal Se deficiency in livestock.
    • Current analytical methods require improvement to ensure reliable Se determination in the clinically relevant range (0.15-0.5 mumol/L).
    • Further validation and standardization of analytical protocols are necessary for consistent selenium status assessment in cattle.