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Rapid determination of methyl mercury in fish and shellfish: collaborative study
Journal - Association of Official Analytical Chemists
|July 1, 1987
Summary
A modified AOAC method for methyl mercury analysis in seafood was validated across 8 labs. This reliable method accurately quantifies methyl mercury in fish and shellfish, achieving interim official first action status.
Area of Science:
- Analytical Chemistry
- Food Safety
- Environmental Science
Background:
- Methyl mercury is a neurotoxin commonly found in fish and shellfish.
- Accurate quantification of methyl mercury is crucial for food safety and public health.
- Existing methods for methyl mercury determination require validation for routine use.
Purpose of the Study:
- To evaluate a modified AOAC method for methyl mercury determination in fish and shellfish.
- To assess the method's reproducibility, accuracy, and recovery across multiple laboratories.
- To gain official approval for the validated method.
Main Methods:
- A modified AOAC method involving HCl extraction and toluene separation was employed.
- Methyl mercury was quantified using electron capture gas chromatography.
- Collaborative study involving 8 laboratories analyzing tuna, swordfish, and oysters at various methyl mercury levels.
Main Results:
- Reproducibility coefficients of variation ranged from 4% to 15%.
- Accuracy, compared to reference values, was between 92% and 101%.
- Recovery from fortified samples ranged from 86% to 98%.
Conclusions:
- The modified AOAC method provides accurate and reproducible determination of methyl mercury in fish and shellfish.
- The method is suitable for routine analysis and has been approved interim official first action.
- This validated method enhances seafood safety monitoring capabilities.