Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Information-rich interferometric point-spread function for type-B 4Pi localization microscopy.

Optics express·2025

Same author

Estimating Maximal Aerobic Speed in Academy Soccer Players: A Comparison Between Time Trial Methods and the 30-15 Intermittent Fitness Test.

European journal of sport science·2025

Same author

The effect of knee-flexion angle on peak force and muscle activation during isometric knee-flexor strength testing using the Nordbord device in soccer players.

Science & medicine in football·2025

Same author

A C4 plant K+ channel accelerates stomata to enhance C3 photosynthesis and water use efficiency.

Plant physiology·2025

Same author

In Depth Mapping of Mesoporous Silica Nanoparticles in Malignant Glioma Cells Using Scattering-Type Scanning Near-Field Optical Microscopy.

Chemical & biomedical imaging·2024

Same author

Intravital Microscopy With an Airy Beam Light Sheet Microscope Improves Temporal Resolution and Reduces Surgical Trauma.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2024

Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026

Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026

Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026

Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026

Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026

Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Aug 25, 2025

Determining 3D Flow Fields via Multi-camera Light Field Imaging

Determining 3D Flow Fields via Multi-camera Light Field Imaging

Published on: March 6, 2013

Snapshot volumetric imaging with engineered point-spread functions.

Daniel Olesker, Andrew R Harvey, Jonathan M Taylor

|October 15, 2022

Summary

This summary is machine-generated.

This study introduces 3D engineered point-spread function microscopy (3D-EPM) for high-resolution, 3D biological imaging. This new method captures rapid cellular dynamics in real-time without sacrificing spatial or temporal resolution.

More Related Videos

Echo Particle Image Velocimetry

Echo Particle Image Velocimetry

Published on: December 27, 2012

High-speed Particle Image Velocimetry Near Surfaces

High-speed Particle Image Velocimetry Near Surfaces

Published on: June 24, 2013

Related Experiment Videos

Last Updated: Aug 25, 2025

Determining 3D Flow Fields via Multi-camera Light Field Imaging

Determining 3D Flow Fields via Multi-camera Light Field Imaging

Published on: March 6, 2013

Echo Particle Image Velocimetry

Echo Particle Image Velocimetry

Published on: December 27, 2012

High-speed Particle Image Velocimetry Near Surfaces

High-speed Particle Image Velocimetry Near Surfaces

Published on: June 24, 2013

Area of Science:

Biophysics
Microscopy
Cell Biology

Background:

Biological processes involve complex 3D interactions at high speeds.
Existing 3D imaging techniques often compromise spatial or temporal resolution.
2D imaging requires plane-by-plane acquisition, limiting speed, while snapshot methods reduce resolution.

Purpose of the Study:

To develop a 3D microscopy technique enabling snapshot imaging of extended biological structures.
To maintain native microscope resolution in both space and time for 3D imaging.
To introduce a computational recovery strategy for volumetric reconstruction from 2D images.

Main Methods:

Introduction of 3D engineered point-spread function microscopy (3D-EPM).
Development of a computational recovery strategy for 3D image reconstruction from 2D EPM images.
Validation on point-like and extended biological samples.

Main Results:

3D-EPM enables snapshot 3D imaging with retained spatial and temporal resolution.
Successful volumetric reconstruction of arbitrary 3D structures from 2D raw images.
Demonstrated imaging of intracellular chloroplast motion (cyclosis) in Egeria densa.

Conclusions:

3D-EPM offers a generalized computational methodology for 3D image recovery.
The technique is adaptable to various microscopy platforms and engineered point-spread functions.
Expected broad applicability in studying rapid 3D biological dynamics.