Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Detection of heritable mutations as quantitative changes in protein expression.

C S Giometti, M A Gemmell, S L Nance

    The Journal of Biological Chemistry
    |September 15, 1987
    PubMed
    Summary

    This study used two-dimensional electrophoresis to detect heritable mutations in mice by analyzing protein expression changes. Computer analysis identified mutations reflected as decreased protein abundance, confirming the technique

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Progesterone-dependent sexual behavior and protein patterns in the ventromedial hypothalamus of the adult female rat.

    Molecular and cellular neurosciences·2009
    Same author

    Identification of membrane proteins in the hyperthermophilic archaeon pyrococcus furiosus using proteomics and prediction programs.

    Comparative and functional genomics·2008
    Same author

    Structural modifications of Methanococcus jannaschii flagellin proteins revealed by proteome analysis.

    Proteomics·2001
    Same author

    Methanol toxicity and formate oxidation in NEUT2 mice.

    Archives of biochemistry and biophysics·2001
    Same author

    Mouse liver selenium-binding protein decreased in abundance by peroxisome proliferators.

    Electrophoresis·2000
    Same author

    A comparison of liver protein changes in mice and hamsters treated with the peroxisome proliferator Wy-14,643.

    Electrophoresis·1998

    Area of Science:

    • Genetics
    • Biochemistry
    • Toxicology

    Background:

    • Heritable mutations can arise from environmental exposures.
    • Detecting mutations at the molecular level is crucial for understanding genetic damage.

    Purpose of the Study:

    • To investigate the utility of two-dimensional electrophoresis in detecting heritable mutations.
    • To assess quantitative changes in protein expression as indicators of mutation.

    Main Methods:

    • Mice sires were exposed to gamma radiation or ethylnitrosourea.
    • Liver proteins from offspring were analyzed using two-dimensional electrophoresis and image analysis.
    • Computerized searches identified changes in protein abundance and presence.

    Main Results:

    Related Experiment Videos

    • Heritable mutations were detected as new proteins or decreased abundance of existing proteins.
    • Ethylnitrosourea treatment led to new proteins with corresponding diminished nearby spots.
    • Computer analysis successfully identified mutations based on decreased protein abundance.

    Conclusions:

    • Two-dimensional electrophoresis is effective for detecting mutations indicated by quantitative protein expression changes.
    • Protein stability is a key factor for reliable mutation detection using this method.
    • This technique aids in identifying genetic damage from mutagenic exposures.