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Updated: Aug 25, 2025
Monitoring of Nanodrug Accumulation in Murine Breast Cancer Metastases
Published on: August 23, 2024
Mesoporous Silica Nanoparticles Loaded with Resveratrol Are Used for Targeted Breast Cancer Therapy
Yifan Gu1, Zhewei Fei1,2
1Department of Breast Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
Objective:
The characteristics of poor pharmacokinetics, stability, and low solubility seriously limited the clinical application of resveratrol (Res) in breast cancer. Thus, this study intends to develop a delivery system for Res which could be better used in breast cancer therapy.
Methods:
Resveratrol-modified mesoporous silica nanoparticles (MSN-Res) were chemically constructed. Their shape and encapsulation were detected by transmission electron microscope, Fourier transforms infrared spectrometer, and UV spectroscopy, respectively. MGF-7 tumor-bearing mice were established by subcutaneous injection, and the pathological changes were detected by hematoxylin-eosin staining. CCK-8 and Ki-67 immunohistochemical staining were used for proliferation evaluation in vitro and in vivo. Flow cytometry, TUNEL, wound healing, and transwell assay detected cell apoptosis, invasion, and migration.
Results:
MSN-Res was successfully prepared with high biosafety. MSN-Res inhibited MGF-7 cell proliferation, invasion, and migration and promoted apoptosis in vitro. Furthermore, MSN-Res showed better performance compared Res in breast cancer mouse models. In addition, we found that MSN-Res inhibited tumor growth via inhibiting the NF-κB signaling pathway.
Conclusion:
MSN-Res inhibited breast cancer progression with better efficacy compared with Res treatment alone by inhibiting the NF-κB signaling pathway, suggesting that MSN-Res is a more effective adjuvant treatment method for breast cancer. Thus, our findings may provide a new and safer means of using phytochemicals in combinatorial therapy of breast cancer.
Insights
This study developed resveratrol-modified mesoporous silica nanoparticles (MSN-Res) to improve breast cancer treatment. MSN-Res demonstrated enhanced efficacy in inhibiting tumor growth and progression compared to resveratrol alone.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Cancer Therapy
Background:
- Resveratrol (Res) has limitations in breast cancer treatment due to poor pharmacokinetics, stability, and solubility.
- Developing effective delivery systems is crucial for enhancing Res's therapeutic potential.
Purpose of the Study:
- To develop and evaluate resveratrol-modified mesoporous silica nanoparticles (MSN-Res) for improved breast cancer therapy.
- To investigate the efficacy of MSN-Res in vitro and in vivo models of breast cancer.
Main Methods:
- MSN-Res were synthesized and characterized using electron microscopy and spectroscopy.
- In vitro studies involved CCK-8, Ki-67 staining, flow cytometry, TUNEL, wound healing, and Transwell assays.
- In vivo studies utilized MGF-7 tumor-bearing mice, with efficacy assessed via pathological changes and tumor growth inhibition, including analysis of the NF-κB signaling pathway.
Main Results:
- MSN-Res were successfully prepared with high biosafety.
- MSN-Res significantly inhibited MGF-7 cell proliferation, invasion, and migration while promoting apoptosis in vitro.
- MSN-Res demonstrated superior performance over Res alone in breast cancer mouse models, inhibiting tumor growth by targeting the NF-κB signaling pathway.
Conclusions:
- MSN-Res represent a promising delivery system that enhances resveratrol's efficacy in inhibiting breast cancer progression.
- MSN-Res show potential as an effective adjuvant therapy for breast cancer, offering a safer approach for phytochemicals in combinatorial treatments.
