Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

7.4K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.4K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

6.4K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.4K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

2.7K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.7K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

3.8K
The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
3.8K
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

5.9K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
5.9K
cAMP-dependent Protein Kinase Pathways01:25

cAMP-dependent Protein Kinase Pathways

6.5K
Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
6.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Construction of a Nomogram Prediction Model for Mortality Risk Within 14 Days in Patients with Acute Myocardial Infarction and Ventricular Septal Rupture.

Journal of clinical medicine·2026
Same author

Posterior circulation hypoperfusion plays an important role in refractory otalgia: case report.

BMC surgery·2026
Same author

Intravascular ultrasound-guided percutaneous coronary intervention in acute coronary syndrome stratified by the TVF-ACS risk score: the IVUS-ACS trial.

European heart journal open·2025
Same author

A Real-World Pharmacovigilance Study of Fruquintinib Based on the FDA Adverse Event Reporting System (FAERS) Database.

Cancer medicine·2025
Same author

GRK2 Orchestrates VSMC Phenotypic Modulation via DNMT1-Mediated DNA Methylation Reprogramming.

Arteriosclerosis, thrombosis, and vascular biology·2025
Same author

Emerging immunotherapies in osteosarcoma: from checkpoint blockade to cellular therapies.

Frontiers in immunology·2025

Related Experiment Video

Updated: Aug 25, 2025

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

7.0K

PCAF Accelerates Vascular Senescence via the Hippo Signaling Pathway.

Chaohua Kong1, Dongchen Wang1, Feng Wang1

  • 1Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.

Oxidative Medicine and Cellular Longevity
|October 17, 2022
PubMed
Summary
This summary is machine-generated.

P300/CBP-associated factor (PCAF) drives endothelial cell senescence by impacting the Hippo signaling pathway. Reducing PCAF in aged mice lessened vascular aging, suggesting PCAF as a therapeutic target for vascular aging.

More Related Videos

Culturing and Manipulation of O9-1 Neural Crest Cells
08:32

Culturing and Manipulation of O9-1 Neural Crest Cells

Published on: October 9, 2018

10.2K
Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor
07:16

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor

Published on: September 13, 2018

20.0K

Related Experiment Videos

Last Updated: Aug 25, 2025

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

7.0K
Culturing and Manipulation of O9-1 Neural Crest Cells
08:32

Culturing and Manipulation of O9-1 Neural Crest Cells

Published on: October 9, 2018

10.2K
Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor
07:16

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor

Published on: September 13, 2018

20.0K

Area of Science:

  • Vascular Biology
  • Cellular Senescence
  • Epigenetics

Background:

  • P300/CBP-associated factor (PCAF) is a histone acetyltransferase involved in cell growth and differentiation.
  • PCAF has been implicated in atherosclerosis, but its role in cellular senescence is unknown.
  • Endothelial dysfunction contributes to vascular aging.

Purpose of the Study:

  • To investigate the role and mechanism of PCAF in endothelial cellular senescence.
  • To explore PCAF's potential as a therapeutic target for vascular aging.

Main Methods:

  • Immunohistochemistry (IHC) in aged mice aorta.
  • In vitro studies using human umbilical vein endothelial cells (HUVECs) treated with palmitate acid or X radiation.
  • PCAF knockdown and overexpression experiments in HUVECs.
  • Analysis of Yes1 Associated Transcriptional Regulator (YAP) activity.
  • In vivo studies using adeno-associated virus for endothelial-specific PCAF downregulation in aged mice.

Main Results:

  • PCAF expression was increased in aged mouse aortas.
  • Palmitate acid or X radiation induced PCAF and senescence markers in HUVECs.
  • PCAF modulation affected senescence phenotypes in HUVECs.
  • YAP activity was crucial for PCAF-mediated senescence, and its decline was observed in aged vessels.
  • Endothelial-specific PCAF downregulation in aged mice reduced vascular senescence.

Conclusions:

  • PCAF mediates endothelial cellular senescence, partly through the Hippo signaling pathway involving YAP.
  • PCAF is a potential therapeutic target for preventing and treating vascular aging.