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Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Updated: Aug 25, 2025

Three-dimensional Optical-resolution Photoacoustic Microscopy

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

Enhancing optoacoustic mesoscopy through calibration-based iterative reconstruction.

Urs A T Hofmann^1,2, Weiye Li^1,2, Xosé Luís Deán-Ben^1,2

¹Institute for Biomedical Engineering and Institute of Pharmacology and Toxicology, Faculty of Medicine, University of Zurich, Switzerland.

|October 17, 2022

Summary

This study introduces a new method to improve optoacoustic mesoscopy imaging by experimentally capturing the total impulse response. This technique enhances spatial resolution and depth uniformity for clearer visualization of subsurface tissues.

Keywords:

Biomedical imaging Impulse response Iterative inversion Optoacoustic mesoscopy Photoacoustic imaging Quantitative reconstruction Skin imaging

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Last Updated: Aug 25, 2025

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Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging

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Universal Hand-held Three-dimensional Optoacoustic Imaging Probe for Deep Tissue Human Angiography and Functional Preclinical Studies in Real Time

Universal Hand-held Three-dimensional Optoacoustic Imaging Probe for Deep Tissue Human Angiography and Functional Preclinical Studies in Real Time

Published on: November 4, 2014

Area of Science:

Biomedical Optics
Medical Imaging
Acoustic Imaging

Background:

Optoacoustic mesoscopy offers high resolution and optical contrast at depths inaccessible to microscopy.
Current reconstruction methods often use inaccurate models, limiting imaging performance.
Accurate modeling of signal generation, propagation, and detection is crucial but challenging.

Purpose of the Study:

To improve optoacoustic mesoscopy imaging performance by addressing limitations in current reconstruction techniques.
To develop a method for accurately capturing the total impulse response (TIR) without complex simulations.
To enhance spatial resolution and depth uniformity in mesoscopic imaging.

Main Methods:

Proposed a novel approach to experimentally capture the total impulse response (TIR) by scanning a sub-resolution sized absorber.
Implemented this TIR calibration within image reconstruction algorithms.
Validated the method by imaging subcutaneous murine vasculature and human skin in vivo.

Main Results:

Achieved significant improvements in spatial resolution and depth uniformity over a 3 mm range.
Outperformed traditional delay-and-sum and model-based reconstruction methods.
Demonstrated successful in vivo imaging of biological tissues.

Conclusions:

The proposed experimental calibration and reconstruction paradigm enhances optoacoustic mesoscopy.
This method facilitates quantitative imaging while avoiding complex physics-based simulations.
The approach is adaptable to other imaging modalities reliant on TIR-based reconstructions.